Question

You wish to determine the sequence of a short peptide. Cleavage with trypsin yields three smaller peptides with the sequences Leu–Glu, Gly–Tyr–Asn–Arg, and Gln–Ala–Phe– Val–Lys. Cleavage with chymotrypsin yields three peptides with the sequences Gln–Ala–Phe, Asn–Arg–Leu–Glu, and Val–Lys–Gly–Tyr. What is the sequence of the intact peptide?