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Consider a phosphate buffer made from Sodium phosphate monobasic (NaH2Po4) and Sodium phosphate dibasic (Na2HPO4).

a. State the equilibrium and ka for the buffer system. Which phosphate species are at equilibrium? Which one acts as a conjugated base?

b. If you mix 25 ml of 0.25 Sodium phosphate dibasic (NaHPO4) with 50 ml of 0.1M Sodium phosphate monobasic (NaH2PO4) what will be the resulting pH?

c. If you add 2.7 ml of 0.5M NaOH to the solution in (b), what will be the pH?

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Hubert Koch
Hubert KochLv2
30 Sep 2019

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Determine the quantity of each chemical that you are going to use for preparing the buffer. Calculate the amount you will need to weigh out.

Stock Solutions to Prepare:
a.) 0.5 L of 0.2 M monobasic sodium phosphate (A)
b.) 0.1 L of 0.2 M dibasic sodium phosphate (B) (sometimes precipitation is observed over time so this should be made freshly)
c.) 100 mL of 1 M Tris base pH 8
d.) 250 mL of 4 M NaCl
e.) 20 mL of 4 M imidazole
f.) 10 mL of 0.5 M MgCl2

Estimate Buffer pH Adjustment
To calculate the amount of acid needed to adjust the pH of the Tris base (pKa = 8.3) to pH 8 use the Henderson-Hasselbalch Equation.

A.
If you add 1ml of 1M HCL to 100ml of 0.045M Tris Base. What will be the resulting ph? (Pka of tris = 8.3

B.how many grams of tris base and how many mL of 1m HCL would you need to make 700mL of a 0.8M Tris buffer at ph 7.6. The pjs of Tris is 8.3.

C.
How many grams of Na2HPO4 (141.96g/Mol) and NaH2PO4(119.96g/mol) are needed to make 700ml of 0.4m phosphate buffer at ph 6.3? The pka of phosphate is 7.21

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