Question

Although graphical methods are employed for accuratedetermination of the V_max and K_m of anenzyme-catalyzed reaction, sometimes these quantities can bequickly estimated by inspecting values of the initial rates, v, atincreasing [S]. Estimate the V_max and K_mvalues of the enzyme-catalyzed reaction for the following datashown in the columns below:

[S] (M)	v0, (moles/min/)
2.5 x 10-6	28
4.0 x 10-6	40
1 x10-5	70
2 x 10-5	95
4 x 10-5	112
1 x 10 -4	128
2 x 10-3	139
1 x 10-2	140