BIO130H1 Lecture Notes - Lecture 8: Garland Science, Ultracentrifuge, Spliceosome

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16 Oct 2011

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BIO130H1 Full Course Notes

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Binds to green and stops rna polymerase ii from working. Roger kornberg - nobel prize in chem in 2006. Green - similar btw eukaryotic and prokaryotic rna polymerase. Also read chapter 8 pp 544-546 on dna amplification (lab 3) Exons = transcribed, may or may not be translated. Exon in mrna, might not code for protein (regulatory) Exon not in mrna; mrna is just one kind of rna and only mrna makes protein. In yeast cells, 90% of the genes produce mrna -> protein. Exact number of human is not determined but it is similar. That means 10% of genes produce rna as the final product mrna is only 3-5% of total rna. The building blocks of life page 4 each of these rnaps is a multi-subunit protein each is responsible for transcription of different rnas. Page 340 in textbook (point 1 and 2) Introns removed; not primary transcript; 5" cap and poly -a tail.

Related Questions

Does the process occur in prokaryotes, Eukaryotes, or both?

1) RNA synthesis is blocked by actinomycin

2) A single RNA polymerase transcribes genes that encode mRNAs, tRNAs, and rRNA.

3) Transcription of mRNA is blocked by a-amanitin

4) Sigma subunit detaches from RNA polymerase shortly after transcrition has initiated

5) The 5' end of the mature mRNA beings with triphosphate

6) The primary mRNA transcription is inactive

7) Terrmination of transcription requires the protein p factor

1. There are viruses, called bacteriophage, that infectbacterial cells. Following viral entry, the cell begins tosynthesize viral DNA and viral proteins, leading to the productionof mature virus and the death of the bacterial cell. During thecourse of viral infection, transcription of bacterial genes isturned off so that transcription of viral genes predominates. Whichof the following is the most likely explanation for how the virusmanages to shift transcription from bacterial genes to its owngenes?

a. it synthesizes a viral RNA polymerase that recognizes onlyviral promoters

b. it synthesizes a viral DNA polymerase that recognizes onlyviral origins

c. it synthesizes a viral sigma subunit that directs thebacterial RNA polymerase to viral promoters

d. it has special viral ribosomes that only translate viralproteins

2. Unlike bacterial transcripts, eukaryotic RNA transcripts arechemically modified because:

a. Chemical groups help protect the mRNAs from degradation inthe eukaryotic cytoplasm, bacterial mRNAs are not degraded

b. Chemical groups facilitate the transport of eukaryotic RNAsfrom the nucleus to the cytoplasm, bacterial RNAs are synthesizedin the cytoplasm

c. Eukaryotic RNA polymerases cannot transcribe genes unlessthey are chemically modified, bacterial RNA polymerases transcribeunmodified genes

d. Transcripts in eukaryotic cells contain non coding regionsthat must be removed, bacterial transcripts contain only codingsequence

e. b and d

3. Termination of transcription in both E. coli and eukaryoticcells occurs because

a. there is a termination signal in the DNA following the genesequence.

b. there is a hairpin structure followed by a string of U

copperturtle968

1. What is a template strand? A coding strand? 2. What enzyme synthesizes mRNA. 3. How many RNA polymerases do Bacteria have? Eukaryotes? 4. What is a sigma? How is it related to the RNA polymerase? 5. What is required for transcription to begin? 6. Why are the several different types of sigma?

BIO130H1 Lecture Notes - Lecture 8: Garland Science, Ultracentrifuge, Spliceosome

BIO130H1 Full Course Notes

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