MGY299Y1 Lecture : Notes taken during lecture
Document Summary
Can pass from one species to another; from one bacterium to another. Broad host ranges self-conjugative plasmids have. Needs conjugative plasmid that codes for pillus: ex. F factor: finds receptor on f-, draws the two bacteria together so cell walls touch then genetic material passes through. In our hfr experiment, screen for five different factors. Mechanism: single stranded dna goes into recipient. complementary strand made in both donor and recipient. Requires tra genes, encoded on conjugative plasmid. Large plasmid f: single copy, tra last genes to be transferred. Important in hfr transfer because entire bacteria chromosome come between the tra genes and the ones that are transferred before tra genes y. In these cases, tra genes not likely to be transferred: room on plasmid and in the genetic mechanism of transfer for other genes to be carried. same is element present on conjugative plasmid can also be present on chromosome of the recipient bacterium y.