PHGY 209 Study Guide - Final Guide: Gel Electrophoresis, Unified Atomic Mass Unit, Ultracentrifuge
15 Feb 2018
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Both ecf and icf (inside blood cells) Can be studied in vivo (in a living organism) or in vitro (in a test tube: normovolemia = normal blood volume, hypo/hypervolemia = lower/higher blood volume. Functions: transport, act as acid/base balance, protection against bacteria/viruses. Ions including na+, k+, ca2+, mg2+, cl-, hco3- and po4- (see topic i) Proteins (7g% or 7g/100ml) = composed of albumins, globulins, fibrinogen. Ie gel electrophoresis: rate of migration dependent on charge type/distribution and weight of each protein, larger proteins and positively charged molecules would stay behind, stain and analyse. In renal disease: the front peak would decrease. Albumin the smallest and easiest to be lost. Globulin: varies, 90-800k, 2. 7g%, 5 mm hg. *note: all plasma proteins produced from liver, except globulin which come from lymphoid tissue. The compositions of icf and ecf varies (refer to topic i ionic composition) Filtration = tend to push out fluid from capillaries, vs.
