BIO230H1 Study Guide - Final Guide: Reticle, Microscope Slide, Knurling
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BIO230H1 Full Course Notes
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Lab 4- live cell imaging: set to bright field to illuminate. Focusing stage micrometer allows proper visualization of the specimen. Switch to 10x objective to increase magnification: closing the field iris diaphragm reduces the amount of light that shines through, focusing the edge of the field iris diaphragm allows clear visualization of the specimen. When done correctly, the condenser should be near the top of its. Procedure- explanation- view under the microscope. traveling range. until the point the image begins to darken: adjust the condenser iris diaphragm. The condenser iris diaphragm should be closed until the best contrast and resolution have been obtained. Close the diaphragm just: switch to the 40x objective lens. Adjust so that the correct amount of light is allowed through: the condenser iris diaphragm is used to collect and focus the light from the illumination source.