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BCH210H1 Study Guide - Hi-Nrg, Pyruvate Dehydrogenase, Acetyl-Coa

Course Code
Michael Baker

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BCH210H © Lisa Zhao 2012 | Page 1
Lecture 27: Glycolysis
during muscle contraction, ATP is converted to ADP
NRG is used
chemical NRG converted to mechanical NRG
ATP level falls as NRG is used in contraction
need to replenish the ATP
phosphocreatine are phosphate donors that supply ATP
PCr are in higher levels in muscle
PCr supports ATP by giving its P to ADP to make ATP
this rxn is favourable bc PCr has a higher ΔG of hydrolysis than γ-P in ATP
when PCr is diminished, need glucose from blood
glucose enters muscle cell by GLUT4 (transporter)
GLUT4 allows glucose to flow down its conc gradient
glucose is used to produce NRG
glucose is used to support glycolysis (breaking of sugar in the muscle cytoplasm)
aerobic glycolysis breaks glucose into 2 3-C pyruvate molecules
pyruvate is joined by ATP production from glycolysis and NADH is generated
pyruvate then goes into mitochondria through pyruvate transporters
inner membrane of mitochondria has the transport fxs
outer membrane has pores
pyruvate participates mitochondrial oxidation rxns
pyruvate dehydrogenase (PDH) complex (a multienzyme complex) takes CO2 away from pyruvate,
leaving an acetyl group (2-C) and NADH
NADH is used later to make ATP
acetyl coA enters Krebs cycle
2CO2 released, 3 NADH, 1 FADH2 produced
electrons of NADH and FADH2 are used in electron transport to give ATP through oxidative
NADH and FADH2 generate a lot of ATP
glycolysis is an NRG conversion pathway
occurs in cytoplasm of most cells in the body
glycolysis has 10 enzymes associated w it
to break down glucose into the 3-C pyruvates
2 major stages
ATP-investmentenzymes 1-5
need to invest ATP before getting a lot back
energize glucose w ATP
hexokinase is not specific to glucose (broad specificity)
α-D-glucose, mannose, fructose
ATP is transferring P to glucose (favourable rxn)
rxn is irreversible
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