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CSB428H1 : Article #1 Summary

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crimsonwombat705
24 Dec 2010
School
UTSG
Department
Cell and Systems Biology
Course
CSB428H1
Professor
U.Tepass

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Document Summary

Loss of exocyst sec5, sec6 and sec15 in fly epithelia results in de-cad/arm accumulating in enlarged rab11 recycling endosome (re). rab11 interact with sec15 and arm interacts with sec10, as sec15 and sec10 are located on re. The exocyst complex is important for recognition and tethering between vesicle and pm for polarized transport to apical and basal membranes. crb-sdt, baz-par6-apkc, basolateral dlg, scrib and fas3 was unaffected in sec5, but de-cad, arm and d -cat accumulated in apical cytoplasm and around nucleus in punctate structures. De-cad and arm accumulate in enlarged re in sec5. golgi marker lva, ee marker rab5 and le marker hrs were normal, but rab11 was distributed in enlarged punctate structures with higher fluorescence. Some of these large re were formed by coalescence of smaller punctate structures. Additional re marker was also found in higher levels. de-cad and arm colocalized with re in wt cells.

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Related Questions

2. Before nuclear pore complexes were well understood, it wasunclear whether nuclear proteins diffused passively into thenucleus and accumulated there by binding to residents of thenucleus such as chromosomes, or were actively transported andaccumulated regardless of their affinity for nuclearcomponents.
A classic experiment that addressed this problem used several formsof radioactive nucleoplasmin, which is a large pentameric proteininvolved in chromatin assembly. In this experiment, either theintact protein or the nucleoplasmin heads, tails, or heads with asingle tail were injected into the cytoplasm or into the nucleus offrog oocytes. All forms of nucleoplasmin, except heads, accumulatedin the nucleus when injected in the cytoplasm, and all forms wereretained in the nucleus when injected there.
A. What portion of the nucleoplasmin molecule is responsible forlocalization inthe nucleus?
B. How do these experiments distinguish between active transport,in which a nuclear localization signal triggers transport by thenuclear pore complex, and passive diffusion, in which a bindingsite for a nuclear component allows accumulation in thenucleus?

Write true or false and please explain

30. In secretory cells, the constitutive and regulatory pathways ofexocytosis diverge at the cis Golgi network. ------------------ ( )( )
31. Many proteins are posttranslationally glycosylated at tyrosineresidues in the ER.------------------------------------------------- ( ) ( )
32. Cells import LDL molecules exclusively through pinocytosis.-------------------------------------------------------------------------( ) ( )
33. Cells recycle LDL receptors from lysosome to the plasmamembrane.----------------------------------------------------------------- () ( )
34. Phagosomes fuse with late endosome.------------------------------------------------------------------------------------------------------( ) ( )
36. If a Sec mutant showed an accumulation of newly synthesizedproteins at ER,
then the Sec gene is likely a component of vesicle-buddingmachinery of trans-Golgi network.------------------------------------ ( ) ( )
37. A signal recognition particle is a single pass trans-membraneprotein of the ER membrane.---------------------------------------- ( ) ( )

Select ALL of the true statements about protein targeting.

Nucleotides interact with a nuclear import receptor for transport into the nucleus for the process of DNA replication and transcription.

Signal sequences that direct proteins to the correct compartment are added to proteins through post-translational modification.

The SRP receptor functions to maintain the position of the ribosome on the ER membrane during the process of protein sorting to the ER.

Introduction of non-hydrolyzable GTP into a cell would cause an accumulation of importin receptors inside the nucleus.

all of the above are true

none of the above are true