Anatomy and Cell Biology 3309 Midterm: Lab 11 - Blood & Blood Formation
22 May 2018
School
Department
Professor
Histology 3309
Lab 11
Blood and Blood Formation
Identify the structure indicated by the arrow
- Lymphatic vessel
- Lining of endothelial cells
o No tunica media or adventitia
- Irregularly shaped wall
What layer of the heart is this a section of?
- Myocardium
- Find cardiac muscle here
- Centrally located nucleus
- Perinuclear cytoplasm
Identify the layer indicated by the bracket
- Endocardium lined by the endothelial cells - Find purkinje cells here
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The arrow is pointing to the lumen of what structure? Be specific.
- Muscular artery
o Very prominent internal and external elastic lamina (identify by this)
o May see some remnant of elastic
- The one on top is an elastic artery
Blood Smear
- Should know whether were looking at a blood smear or a bone marrow smear
o Blood smear: waaay more anucleated cells
o Bone marrow smear: cells at many different stages
Erythrocyte (RBC)
- 7-8microm (use as a scale for size and staining properties)
o bc they stain for eosinophilia you can use the intensity of stain for RBC to differentiate
bw eosinophils, neutrophils etc.
- biconcave shaped: thin at center
o see some transparency in the center with a darker halo around the edges
- EM: see dumbbell shape
- Anucleated
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find more resources at oneclass.com
Agranulocytes: monocyte
- Very large cell (green circle)
- 12-20 micron
o more than double the size of RBC
- very pale cytoplasm
- horse shoe shaped nucleus
arganulocytes: lymphocytes
- same size as RBC (green circle)
- darkly stained, basophilic nucleus with very little cytoplasm
- may identify by just a floating nucleus
Leukocytes: neutrophil
- leukocytes are WBCs
- neutrophils have pale staining cytoplasm
- 10-15 microm in diameter (slightly smaller than monocytes)
- have a multilobe nucleus (3-5 lobes)
o THESE ARE NOT MULTINUCLEATED, THEY ARE MULTILOBED NUCLEI
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Lining of endothelial cells: no tunica media or adventitia. Endocardium lined by the endothelial cells - find purkinje cells here. Muscular artery: very prominent internal and external elastic lamina (identify by this, may see some remnant of elastic. The one on top is an elastic artery. Should know whether were looking at a blood smear or a bone marrow smear: blood smear: waaay more anucleated cells, bone marrow smear: cells at many different stages. 12-20 micron: more than double the size of rbc very pale cytoplasm. Horse shoe shaped nucleus arganulocytes: lymphocytes same size as rbc (green circle) Darkly stained, basophilic nucleus with very little cytoplasm. May identify by just a floating nucleus. 10-15 microm in diameter (slightly smaller than monocytes) Have a multilobe nucleus (3-5 lobes: these are not multinucleated, they are multilobed nuclei. Depending on section of cut, if you look at em, you can see the attachment of the lobe.
