Biology 2581B Study Guide - Quiz Guide: Okazaki Fragments, Dna Replication, Nuclear Pore

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3 Jun 2020

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Have one polymerases, b and e three. Rna polymerase: connects complementary rna bases to dna. It needs a promoter to know where to start (dna a primer): sequence of dna where rna can start, not incorporated into final product. Repressors can bind to operator to stop rna polymerase. Dna polymerase: connects complementary dna bases to dna and proofreads the sequence. Ssb: bind to the single strands to keep them separated. Primase: shows dna polymerase where to start by creating a primer. Okazaki fragments: lagging strand has to be replicated in fragments because dna polymerase can"t do 3"-5" synthesis. Primers have to be replaced by dna bases since they"re made of rna. cytoplasm: cytosol + organelles. Dna consists of two antiparallel, complementary strands that are non-covalently attached. Chromosomes are visible as chromosomes during mitosis, not during a specific phase. mrna < trna < rrna. Movement of membrane lipids and proteins: laterally, flexion, rotation. Not from one layer to another (flip flop)

Related Questions

Place the following steps of DNA replication in right chronological order.
1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides

plumsardine151

1) What is the difference between the leading strand and the lagging strand in DNA replication? Place the following steps of DNA replication in right chronological order.

1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides

2) Given the template DNA and primer sequences below, what bases will be added to the primer as DNA replication proceeds? (The bases should appear in the order that they will be added)

5'-GTCGTCTCCCTTTTTAAAAACCCCCCC-3'
5'-UUUUUAAAAAGGG-3'

3) Given the DNA sequence below, what could be a primer sequence used in its replication?
5'-CGTACGGCCCCAAAATTGCTACTCTGCTG-3'

raspberryleopard304

Double check to see if I got these right? 3 questions

Some Processes Involved in DNA Replication

1.The replication origin is identified.

2 DNA primase builds RNA primer.

3Okazaki fragments are spliced by DNA ligase.

4Helicases bind and uncoil the DNA double helix.

5 RNA primer is the starting point for DNA polymerase.

6 DNA replication ends at the telomere where DNA codes fortermination.

7 DNA polymerase adds complementary nucleotides in the 5' to 3'direction in short segments called Okazaki fragments.

The sequence in which the events numbered above occur during DNAreplication of the lagging strand: Number 1 - 7 in order.

I put: 1,4,2,5,7,3,6

__________________Question 2:

Some Functions of Enzymes

Build DNA strands
Build RNA primers
Build mRNA strand
Cleave DNA strands
Splice Okazaki fragments

Enzyme:
Helicase :4
DNA ligase :5
RNA primase :1
RNA polymerase :3
DNA polymerase:2

Question 3:

like mRNA, tRNA has a ribose sugar, U instead of T, and issingle stranded. Unlike mRNA, which remains a long single strand ofnucleotides, tRNA folds so that some areas pair up.

The resulting structure has an anticodon on one end and a site foran amino acid to attach on the other end. There is basecomplementarity (A pairs with U and G pairs with C) between an mRNAcodon and tRNA anticodon.

If the amino acid tyrosine attaches to a tRNA, which of thefollowing anticodons could be at the opposite end of the tRNAmolecule?

Select one:

a. ATA and ATG

b. UAU and UAC

c. AUA and AUG

d. AGA and AGU

I picked B.

purpleturtle13

Biology 2581B Study Guide - Quiz Guide: Okazaki Fragments, Dna Replication, Nuclear Pore

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