BIO SCI 93 Study Guide - Midterm Guide: Scantron Corporation, Deoxyribose, Pyruvic AcidExam


Department
Biological Sciences
Course Code
BIO SCI 93
Professor
Jorge Busciglio Kim Green Marcelo A Wood
Study Guide
Midterm

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Fall 2016 Bio 93 (section A)
Exam 2 (11.7.16)
Version A
Instructions: Write your name, and bubble in your version and student ID
on your scantron (see front of the room for more details).
Write your name here: _________________________________________
This exam contains 22 questions for 200 points total. Each question (4 to
25) is worth 9.1 points. Choose the best answer for each question.
Questions 1 to 3 are survey questions that will not impact your grade in any
way, but please be sure to answer all of them. Please ask questions if you
don’t understand something. When you turn in your scantron, also turn
in the entire exam.
Wait until instructed to begin the exam. Good luck!
Please answer questions 1 through 3 before you answer any other questions on the exam.
1. How many hours did you spend studying for this exam?
A. 0 to 2 C. 6 to 8 E. More than 12
B. 3 to 5 D. 9 to 12
2. When did you start studying for this exam?
A. I didn’t study C. Two days before E. Four or more days before
B. The day before D. Three days before
3. Based on how you studied for this exam, what grade do you think you will earn?
A. 90 to 100% C. 70 to 79% E. Less than 60%
B. 80 to 89% D. 60 to 69%

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Chitin is the second most abundant polysaccharide in nature and is found in arthropods,
fungi, and other organisms. Chitinase, an enzyme that hydrolyzes chitin, is produced by
many bacterial species for nutritional purposes. Use this information to answer the following
three questions.
4. ATP is required for chitinase to function properly. Which of the following statements about
ATP is TRUE?
A. The hydrolysis of ATP is an endergonic reaction with G < 0
B. Oxygen is required for ATP hydrolysis to proceed
C. ATP hydrolysis powers chemical work by heating the surroundings
D. ATP stores energy in its bonds as kinetic energy
E. ADP and Pi have lower free energy than ATP and water
5. The main function of chitinase is to catalyze the hydrolysis of chitin. Suppose that
chitinase is added to a solution of chitin and ATP in water. What do you expect to occur as
the reaction proceeds?
A. G would decrease, EA would decrease, and the concentration of monosaccharides would
increase
B. G would not change, EA would decrease, and the concentration of monosaccharides would
increase
C. G would increase, EA would increase, and the concentration of amino acids would increase
D. G would not change, EA would decrease, and the concentration of amino acids would
increase
E. G would not change, EA would increase, and the concentration of monosaccharides would
increase
6. Chitinase from the bacterial species
Microbispora was isolated and studied by
researchers. They measured chitinase
activity as a function of pH and
temperature (see figures at right). What
can you conclude from these data?
A. Microbiospora likely live in
environments with low hydrogen ion
concentrations
B. The primary structure of chitinase is
likely unaffected at temperatures
above 70 degrees
C. Chitinase is able to hydrolyze
cellulose very well at pH ~3
D. Chitinase is more active at 30
degrees than at pH ~11
Researchers were investigating the effects of a newly discovered compound, BAM15, on
cellular function. They first started by assessing the effects of BAM15 on cellular respiration.
Use this information to answer the following three questions.
7. Consider a cell that is actively producing energy via cellular respiration. What statement
about this cell is TRUE?
A. The electron transport chain is producing ATP by reducing oxygen to water
B. The mitochondrial intermembrane space is more alkaline than the mitochondria matrix
C. Pyruvate oxidation is producing NADH and FADH2
D. NAD+ is being reduced to NADH in the cytoplasm
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