PSYC 3250 Chapter Notes -Diffusion Mri, Radioactive Tracer, Anterograde Tracing
13 May 2015
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Document Summary
The lesion method, oldest, and it remains one of the most useful. A subcortical lesion is made under the guidance of a stereotaxic apparatus. Injection of molecules of toxic saporin attached to an antibody targeted against a protein found in particular neurons in the brain produces even more specific lesions. 1 the location of a lesion must be determined after the animal"s behavior has been observed (histological method). Animal is killed and the brain is perfused with a saline solution, removed, and placed in a fixative such as formalin. 2 light microscopes enable us to see cells and their larger organelles, but an electron microscope is needed to see small details, such as individual mitochondria and synaptic vesicles. Scanning electron microscopes provide a three-dimensional view of tissue but at a lower magnification than transmission electron microscopes. Confocal laser-scanning microscopes provide images of slices of tissues that can show the presence of particular molecules even in living tissue.
