BIOL 3P30 Lecture Notes - Lecture 27: Gluconeogenesis, Rate-Determining Step, Fructose
22 Aug 2015
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Hk, pfk, pk: we can define these three steps as 3 small pseudocycles, all three pseudocycles have: Opposing reactions are catalyzed by different enzymes. Opposing reactions offer independent control of rate of flux through glycolysis or gluconeogenesis by controlling activity of distinct enzyme. Allosteric enzymes products or coenzymes in the pathway. Enzymes that are activated or inhibited by substrates, Contain n allosteric binding sites for activator or inhibitor. Binding at the allosteric site produces a conformational change in the enzyme that changes. Km for a substrate which is the [s] o giving 1/2vmax (changes the affinity of the enzyme for its substrate) o. Let"s look at v vs [s] graph where the affinity for substrate is modulated. F-6-p +atp fructose 1,6 bisphosphate + adp. Means that activity of enzyme is increased or inhibited by effectors that are substrates, products or co-enzymes of the pathway but not necessarily of the enzyme itself.
