BIOL 300 Lecture Notes - Lecture 18: Nxf1, Exon Junction Complex, Eif4E

• School: McGill University
• Department: Biology
• Course: BIOL 300
• Professor: Frieder Schoeck

Document Summary

Lecture 18: transport of mrnps out of the nucleus. How do rnas move out of the nucleus: first analyzed through electron microscopy in fly called chironomus, you can see that immediately after the rna gets synthesized by rna. Polymerase, it gets associated with rna binding proteins; it is never naked: this entire complex is called hnrnp (heterogeneous nuclear. Structure of the nuclear pore: the nuclear pore is filled with fg nucleoporins: the exportin/importin/rna have to move through all of this, the rna moves through the nuclear pore with the help of proteins: Fg-nucleoporins (most important) mrna export mechanism: protein called dbp5 (name doesn"t matter) is a rna helicase they hydrolyze atp and move along the. By moving along the rna, it displaces the proteins that are bound to the rna, including nxf1 and nxt1: this generates a concentration gradient for the nxf1/nxt1 protein (higher concentration in the nucleus) enables.