MIMM 323 Lecture Notes - Histone H4, Histone Deacetylase, Sirtuin 1
23 Nov 2012
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If you have a haploid strain with mutation in alpha1 (mat alpha1-) No alpha1 produced but alpha2 still produced: haploid-specific genes are on, a-specific genes are off, alpha-specific genes are off. No receptor and no pheromones released sterile strain. Need another protein for expression of alpha and a-specific genes: mcm1, also known as prtf. Binds upstream activating sequences of alpha and a-specific genes. Mcm1 binds with high affinity to p-box upstream of a-specific genes. The p-box binding site for mcm1 has low affinity recognition ( faulty p-box ) In order to bind with high affinity, need secondary alpha1 factor. Alpha1 and mcm1 form complex which binds together to p-box. Prevent binding of mcm1 to p-box of a-specific genes. Alpha2 and alpha1 form a complex (alpha2-alpha1-alpha2) Figure 1: combinations of prtf, alpha1 and alpha2. Expression of mat and repression of hml and hmr on chromosome 3.
