BIOLOGY 2B03 Lecture 5: Lecture 5 Summary

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chocolateprairie-dog409

9 Feb 2012

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Primarily found in secretory protein or on the exoplasmic domain of membrane proteins. Addition of an ogliosaccharide to block the nh2 group from the asn a. a. residue. O-linked = addition of 1-4 sugar residues on the oh group of ser,thr. As polypep enters the er lumen, it is glycosylated by the addition of an ogliosaccharide as an intact unit to the asn a. a. from the lipid called dilichol. Accelerated by er resident proteins: pdis (protein disulphide isomerase, chaperones (hsc70 and bip, lectins binds to unfolded/misfolded polypeptides (carbohydrate adding ones calnexin, peptidyl-propyl-isomerase monitors rotation about the peptide bond. Promotes reliable and rapid folding in proteins in the er and the assembly of single subunits into multisubunits. Cleaving of signal secreted proteins and type 1 integral membrane proteins signal cleaved by signal peptidase. Inter/intra molecular disulphide bonds stabilize the tertiary and quartenary structures of a protein. Oxidative linkages between sh groups on 2 cysteine a. a residues.

Related Questions

Where in the cell is the assembly of N-oligosaccharides on thesecretory and integral membrane proteins initiated? b. Brieflydescribe the mechanism of assembly and attachment of the coresegment of the carbohydrate chain to a typical targetpolypeptide.

greengoldfish40

After the addition of three glucose molecules to the completedoligosacharide precursur (Glc3Man9(GlcNAc)2), which of thefollowing reactions most likely occurs?

		The unfolded protein response
		Folding and retention by lectin domain containing proteins
		The recruitment of hnRNPs
		Protein disulfide isomerase mediated oxidation
		Glycosylation of a new protein which is/has entered the ER lumencatalyzed by oligosaccharyl transferase

roseferret689

12. You have undertaken a study ofprotein transport in live cultured eukaryotic cells, and yourobjective is to follow the in vivo movement of asecreted protein through the secretory pathway to the cellexterior. You have introduced a gene that encodes a mutant form ofthis protein tagged with Green Fluorescent Protein (GFP) into thecells. When the cells are grown at 42^OC, the mutantprotein is folded in a way that causes it to be retained in therough ER, whereas at 32^oC, the protein folds normallyand is able to proceed through the pathway. Briefly describe anexperiment that would allow you to accomplish your objective and besure to mention the microscopic technique you would use.

Where in the cell is the assembly ofN-oligosaccharides on the secretory and integral membraneproteins initiated?
b. Briefly describe the mechanism of assembly andattachment of the core segment of the carbohydrate chain to atypical target polypeptide.

byzantiumhound935

BIOLOGY 2B03 Lecture 5: Lecture 5 Summary

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