MBIO 2020 Lecture Notes - Lecture 5: 40 Minutes, Sigma Factor, Ribosomal Rna
Document Summary
5" to 3" direction towards ter site: dna pol cannot initiate synthesis without a primer, a primer is synthesized by primase (dna dependent rna pol) which makes a short, about. 10 nucleotides, complementary rna starting at ori c. After the leading strand synthesis has proceeded and the rf has moved away from ori, primase synthesizes a new primer rna which is extended by dna pol 3. Direction is towards ori c until it stops - forms a fragment okazaki fragment. As rf moves, another okazaki fragment can be formed: lagging strand is comprised of okazaki fragments, about 1000-2000 bp long, formed discontinuously. The overall replication is semi discontinuous: leading strand - continuous - 1 primer, lagging strand - discontinuous - multiple primers. Dna gyrase - moves in front of rf relieves super twisting by breaking and rejoining of one strand of dna.