MBIO 3010 Lecture Notes - Lecture 16: Retrospective Diagnosis, Medical Microbiology, Molecular Diagnostics

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Identi cation of the microorganism in the patient specimen involved in the disease process. Detection of microorganisms or their products in specimens collected from the patient. Detection of the patients immune response to infection. Provide antimicrobial susceptibility of the isolated microorganism. Designing proper therapy on type of organism - cannot be determined while looking under the microscope. Identi cation of microorganisms by isolation and culture. Area of presence, how much organisms are present vs. how many are supposed to be present under normal conditions. ex) stool, csf. Identi cation of a speci c microbial gene or product. Detection of speci c antibodies to a pathogen. Design primer that is speci c to a speci c bacterial gene - if it doubles then the gene you"re looking for is present. Can use pcr to target resistant gene - see if resistance is present. Always have to check if patient has been vaccinated - will carry antibodies even without being infected.

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