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Lecture 17

Lecture 17 - Transcription - BIOL308.pdf

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BIOL 308
Bruce Wolff

Lecture 17Transcription ProkaryotesEcoli RNA polymeraseGene ExpressionProcess in which information carried by a gene is converted into observable productTranscriptionFirst step in gene expression process where one strand of a DNA molecule is used as a template for synthesis of a complementary RNA mRNA which carries information for a specific proteinGene Expression and Changes in Gene Expression are Controlled RegulatedOnecell system BacteriaHas to survive reproduceGene expression regulationto adjust to changes in its nutritional environment to enable cell growth and cell divisionMulticellular organismHas to survive and reproduce but also to grow and develop different parts of the body different functionGene expression regulationduringDevelopment timeTissue differentiation space and spacetime comboStress as a response to environmental stress inductionRegulation of Gene Expression ProkaryotesProkaryotestranscriptioninitiationis controlledTranscription and translation occur in the same compartmentmRNA ispolycistronic without introns and has a short halflifeRegulation of Gene Expression EukaryotesEukaryotic cell is compartmentalized regulation in each partEukaryotic cell is compartmentalized regulation in each partGene expression can be regulated at various levels in eukaryotesTranscription initiation is most important level of regulationTranscription generalSimilar process to DNA synthesisEnzyme multisubunit complexEnzyme produces nucleotide strand in 5 to 3 direction2Mgis a cofactor necessary to add to buffers for in vitro transcription NOTES FROM LECTURES Page 1
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