CHM 4139 Lecture Notes - Lecture 9: Catalase, Turnover Number, Enzyme
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Which means that they can transform many molecules before they deactivate. Represents the maximum number of substrate molecules converted into product(s) per active site per unit of time. In the simplest kinetic scheme, e + s ! Is the [s] that gives v= vmax. Vmax is achieved at [s] 10 x km. In the simples reaction scheme, e + s ! Km represents an apparent dissociation constant for the michaelis complex, e s. Km reflects the productive binding (how well the substrate bind to the enzyme and how well does he substrate get transformed into products). The lower the km value, the better the substrate. Typically, km values are in the m-mm range, but can be as low as nm. An apparent second order rate constant: at low [s], [s] <