BIOB11H3 Lecture 11: BIOB11 – Lecture 11
Document Summary
Gene regulation ii: transcription factors and microarray technology. Transcription factors (tfs) often to palindromic dna sequences via multiple non-covalent interactions. Two functional domains exist in transcription factors: dna binding and transcriptional activation. There are several classes of tfs, which are usually distinguished by particular protein motifs (eg. the leucine zipper) Depending on the tf, it may function by forming homodimers, and it may form heterodimers with other subunits. Combinatorial control is manifest by formation of heterodimers- many combinations can be made from a small number of tfs. Activation domains often work by recruiting components of the preinitiation complex to the promotor of the gene. Microarray technology has been developed to examine global changes in the transcript profile of two samples (eg. a control cell and one treated with a drug) Dna microarrays and massively parallel sequencing allow simultaneous profiling of the activity of all the genes in an organism or cell type.
