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Dan Riggs (200)
Lecture 10

BIOB11H3 Lecture Notes - Lecture 10: Dna-Binding Domain, Histidine, Structural Change


Department
Biological Sciences
Course Code
BIOB11H3
Professor
Dan Riggs
Lecture
10

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LECTURE 10
Transcription factors
Have 2 functional domains:
o DNA Binding domain: sequence specific interaction often
binds to palindromic DNA sequences and may form dimers
that increase many recognition sites
o Activation domain: must interact with proteins to promote
transcription or repression
Nitrogenous bases have specific function that project into major
groove (H bonds)
o Sequence specificity for protein binding resides in the type
or order of the bases (functional groups)
o Functional groups of nitrogenous bases interact with side
chains of amino acids of TF DNA binding domains to
promote specificity
Zinc Finger TF
o Zn coordinates binding of 2 cysteines and 2 histidine
o Structural change into a loop or finger and that part fits
into major grooves to bind.
Helix-loop-helix Transcription Factors
o Bind as dimers
o Basic domain interacts with DNA to determine sequences
specificity
o Binding regulated by helixes. Combinations bind to
different things: 2 genes can make 2 proteins but 3 sites
can be bound because of merging.
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