CSB327H1 Lecture Notes - Lecture 5: Blastocyst, Inner Cell Mass, Dapi

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Published on 10 Oct 2012
School
UTSG
Department
Cell and Systems Biology
Course
CSB327H1
CSB327 Lecture 5 Notes
- Epithelial cells are polarized
o Sit on a basal lamina
o May be motile during wound repair
o Golgi complex is in apical cytoplasm
o Lateral side forms specialized junctions between cells
- Mesenchymal cells are spindle shaped
o Reside in the ECM
o May be motile
o Golgi complex is at leading edge
o Do not form specialized junctions between cells
- Epithelial-mesenchymal transition (EMT)
o Epithelial cells are released from the surrounding tissue in EMT
Tight junctions, adherens junctions and desmosomal junctions are dissociated
The basal lamina is sometimes dissolved by MMP to be able to transmigrate across
The new mesenchymal cells are emigrating
o Epithelial cells is reached with the formation of basal lamina in MET
o EMT is easier than MET
- Effect of solubilizing ECM molecules on corneal epithelial differentiation
o Epithelium isolated from embryonic ECM
o The basal surface blebs if placed on Millipore filter
o The basal surface continues to bleb after adding albumin, IgG, hyaluronan, Chondroitin
sulphate and heparan sulfate
o The basal surface smoothes out after adding collagens capable of polymerizing, fibronectin,
laminin
- Activation of mesenchymal transformation of epithelia by 3D type I collagen gels
o Enzymatically digested type I collagen cannot polymerize in vitro
o Epithelia placed on 3D type I collagen gel
Epithelial cells spread out on the gel and lay down a new basal lamina
o Epithelia suspended in 3D type I collagen gel
Epithelial cells forms multiple cell layers and undergo EMT
The apical side forms mesenchymal-like cells that break free and migrate within the gel
o Lens epithelium
Crystallins, collagen IV and laminin expression is inhibited
Fibronectin, collagen I, collagen V, α5β1 integrin (major fibronectin receptor) expression
on apical surfaces
- Early stages of mouse development
o Zona pellucida is the fertilization extracellular membrane
Zona pellucida is when the blastocyst is implanted
o Morula stage activates E-cadherin (cell-cell adhesion molecule)
o Inner cell mass is source of embryonic stem cells
Gives rise to two different types of tissue (extraembryonic tissue and embryonic tissue)
o Trophectoderm is responsible for embedding the embryo into the uterine wall
o Epiblast gives rise to the three germ layers and contains the actual embryo
o Primitive streak is the onset of gastrulation of the mesoderm and endoderm
- Embryonic stem cells (ESCs)
o Derived from the inner cell mass of blastocyst stage embryos grown in the presence of LIF (anti-
differentiation factor) form pluripotent aggregates
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Document Summary

Epithelial cells are polarized: sit on a basal lamina, may be motile during wound repair, golgi complex is in apical cytoplasm, lateral side forms specialized junctions between cells. Mesenchymal cells are spindle shaped: reside in the ecm, may be motile, golgi complex is at leading edge, do not form specialized junctions between cells. Epithelial-mesenchymal transition (emt: epithelial cells are released from the surrounding tissue in emt. Tight junctions, adherens junctions and desmosomal junctions are dissociated. The basal lamina is sometimes dissolved by mmp to be able to transmigrate across. The new mesenchymal cells are emigrating: epithelial cells is reached with the formation of basal lamina in met, emt is easier than met. Activation of mesenchymal transformation of epithelia by 3d type i collagen gels: enzymatically digested type i collagen cannot polymerize in vitro, epithelia placed on 3d type i collagen gel.

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