MGY299Y1 Lecture : Notes taken during lecture
9 Jan 2012
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Comparison type questions beware of bacteria versus animal viruses differences, virus-cell interactions also lots of similarities. How measure virus: measure using plaque assay like viable count with bacteria, titre = concentration, must mix virus particles and indicator cells really well. 30 to 300 plaques per plate is reliable. T4 dna virus lyses cell turbid cell culture becomes clear because cells are gone, fragments left. Multiplicity of infection number of viruses per cell. Same outcome regardless of whether cell infected by one or multiple virus particles. Very low likely that only one phage infected one cell. Solid overlay of agar viruses cannot really move can infect cells immediately around initially infected cell. Protein part of phage stays outside of cell, only dna injected . Contractile sheath contracts sheath associated with entry of dna into cell. Like injection: animal viruses do not inject*****, bacteria have big cell walls dna need to go through, human cells do not have cell walls.
