Anatomy and Cell Biology 4429A Lecture Notes - Lecture 9: Signal Peptide, Methotrexate, Isotopic Labeling

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Import of proteins from the cytosol to organelles. Import of proteins from the cytosol to mitochondria. Thylakoids and other proteins are made in the cytosol, and then targeted to specific organelles. The nucleus for example, has the function of protecting the contents of the cell (i. e dna). Lysosomes and peroxisomes protect the cell, and the er provides an isolated oxidizing environment for disulfide bond synthesis. The mitochondria maintain a concentration gradient to synthesize atp, and also concentrate substances in the lumen. Hsp70 acts like a ratchet that pulls the protein into the mitochondria. It is the targeting signal and the protein gradient that is mainly involved in the translocation the transport proteins (tim and top) simply acts as a pore. Targeting to outer or inner membrane, or inter-membrane space generally requires more than one targeting sequences. Peroxisome targeting sequence is the skl signal sequence that is found in the carboxy terminal.

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