HE440 Lecture 8: HE400P Week 3
Document Summary
Fas: an adaptor molecule, fas-associated death domain protein (fadd) dimerizes the death receptors. Leads to activation of procaspase 8 to initiator caspase 8: pathway converges with intrinsic. Necroptosis: necrotic cell death that is programmed. Involves membrane rupture: mediated by the necrosome, activation of death receptor, inhibition of caspase 8 activates ripk3, normally caspase 8 would cleave ripk1 -> apoptosis. In normal cells this is sustained by p53 and target molecule p21waf1: genotoxic signaling via chk1/2 involves many cancer associated genes included h2ax, 53 bp1, brca1, mdc1, and the mre11-rad50-nb21 complex: cells that should die by this method but don"t are at risk of oncogenesis. 500 nmol/l 17-aag, cells were collected and immunoassayed with anti- -tubulin antibody counterstained with 4,6 -diaminidino-2-phenylindole. A, mitotic catastrophe figures showing multiple centrosomes, predominant in mba-md-436 cell line. B, metaphase spreads showing missegregation of chromosomes in hcc 1937 cell line and uacc 3199 cell line.
