BIOL-4610 Lecture Notes - Lecture 15: Nuclear Pore, Nuclear Localization Sequence, In Situ Hybridization

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1 Oct 2018

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Mf, mt, and if = attach to nucleus. Change in shape of these can pull the nucleus open. The nucleus has a lipid bilayer made up of hydrophobic and hydrophilic portions. The outer membrane is continuous with the endoplasmic reticulum which is important for protein synthesis. If and actin help bind the nucleus to the plasma membrane. In order to enter the nucleus they have to have specific key to get through the membrane. W/o it the protein can not enter the nucleus. Nuclear matrix: fibrous network in nucleus that helps provide shape and is where chromosomes attach. The matrix is made out of lamins, an if. Histones help change chromatin into what its supposed to be (expressed or not expressed) In situ hybridization: determines location of certain sequences. Use probe with complementary strand of dna to see where sequences are. The nls sequence is added near the c terminus and helps target protein back into nucleus.

Related Questions

Question 1

Light microscopes

	A.	typically provide more resolution than an electron microscope
	B.	work by reflecting electrons off the surface of an object being studied
	C.	use light and glass lenses to magnify an image

Question 2

In the plasma membrane, the phospholipid heads

	A.	are hydrophilic and face outward toward the aqueous solution on both sides of the membrane
	B.	are hydrophobic and face outward toward the aqueous solution on both sides of the membrane
	C.	are hydrophobic and face inward, shielded from water

Question 3

The nucleus of a cell

	A.	is surrounded by a single layer of membrane.
	B.	contains DNA
	C.	is the primary location of protein synthesis

Question 4

Which location in the cell is unlikely to contain ribosomes or ribosomal subunits?

A.		endoplasmic reticulum
B		plasma membrane
C.		Cytoplasm

Question 5

The main function of the rough endoplasmic reticulum is the production of

A.		membrane and proteins secreted by the cell
B.		mitochondria and proteins secreted by the cell
C.		hydrogen peroxide

Question 6

The Golgi apparatus

A.		is the site of glucose breakdown
B.		stores, modifies, and packages proteins
C.		strings together amino acids to produce proteins

Question 7

The function of mitochondria is

		A. cellular respiration
		B. photosynthesis
		C. lipid synthesis

Question 8

Which of the following statements about the cytoskeleton is false?

		A. The cytoskeleton helps to support cell
		B. Once laid down, the elements of the cytoskeleton are fixed and remain permanently in place
	C.	The cytoskeleton is composed of three types of fibers: microfilaments, microtubules, and intermediate filaments

i need help with this homwork please choose the correct answers and just be claer to read and understand for the slect the apply qusestions too and it is important i realy need the right answers so please be claer not write randomly i really need the correct answers to study for my test so hop you can help me

Question 1

What is the smallest cell you could find?

Question 2

TEMs appear as 3D images

True

False

Question 3

Bacteria are what cell type? Write one word as the answer in lowercase and using no syntax.

Flag this Question

Question 42 pts

ALL eukaryotic cells have which of the following. Click all that apply.

membrane enclosed cell organelles

mitochondria

Question 5

Prokaryotes and eukaryotes both have which if the following cellular components. Click all that apply.

Question 6

I am made of one 60s and one 40s subunit, which organelle am I?

protein

lipid

bacterial ribosome

Question 9

The difference between the rough and smooth ER is

the rough produces lipids where as the smooth does not

the rough has many folds and convolutions where as the smooth does not

the rough has ribosome embedded and the smooth does not

the rough is closer to the cell membrane whereas the smooth is closer to the nucleus

Question 10

The site where many lipids, including cell membrane is produced.

Question 11

Name the organelle: In muscle cells in particular, I would increase in number and size in response to frequent exercise

Question 12

In animal cells, glucose would be mobilized from its storage molecule (glycogen) in this part of the cell.

Question 13

Which of these is associated with the rough ER

production of carbohydrates

production of steroid hormones

production of ATP

production of proteins that are secreted from the cell

Question 14

Which organelle sorts, modifies and stores substances produced on the rough ER. Write two words as the answer in lower case separated by a space.

Question 15

Which organelle is responsible for ATP production. Write one word in the singular as the answer.

Question 16

You inherit all of your mitochondria from your

father

half from your mother and half from your father

your siblings

mother

Question 17

An organelle that contains chlorophyll

Question 18

Microtubules have a variety of roles, which of the options below is NOT one of them?

separate chromosomes

cilia and flagellum movement

tracks for organelle movement

constrict the cell during cell division

Question 19

Made of tubulin subunits

microfilaments

chromosomes

intermediate filaments

microtubules

Question 20

Name the part of the cytoskeleton that is made of two twisted rods of actin. Write one word as the answer in lower case.

Question 21

Which part of the cytoskeleton is made of the same protein as your hair and nails?

microfilaments

microtubules

Intermediate filaments

Question 22

The extracellular matrix is found outside of, but attached to the cell membrane?

True

False

Question 23

Which of the options below is an important function of the extra cellular matrix?

connecting cells together

regulate the movement of substances into and out of the cell

helping cells divide into two cells

keeping the cell in a fixed and ridged shape

Question 24

Which part of the cell wall has a high pectin content?

Question 25

Plants have mitochondria, chloroplasts, a cell wall, and nuclei.

True

False

Question 27

What accounts for 40% of the protein in our bodies?

Question 28

Animal cells have cell walls

True

false

1. Name two similarities and two differences between thecellular processes of importing

protein into the ER and importing protein to the nucleus.

2. What proteins are directly involved in the transportation ofcargo in a clathrin-coated

vesicle?

a. Dynamin

b. Adaptins

c. Cargo receptors

d. t SNARES

3. Suppose a cell was treated with colchiine, a drug causingmicrotubule disassembly.

What would happen within the cell? Why?

Suppose there were a genetic defect in the genes that code forkinesins and dyneins.

What would happen within the cell? Why?

4. Which of the following are true about microtubules?

a. They are utilized by the disease causing bacteriaLysteria.

b. Instability in the minus-end of the microtubule is caused bythe hydrolization of GTP.

c. Centrosomes contain binding sites for microtubules composed atype of tubulin ring.

d. Cellular concentrations of tubulin are above the criticalconcentration for plus-end

polymerization.

e. Motor proteins can attach organelles to the microtubules.

5. Indicate which of the following statements are false:

a. Membrane fusion is energetically unfavorable and thusrequires ATP or similar high

energy molecule to occur

b. Clathrin is responsible for capturing specific molecules fortransport in coated vesicle

structures.

c. Receptor proteins that are incorporated into an endosome canbe retrieved back to the

membrane domain it originated from; can return to a new domainof the membrane; or

can enter the lysosome for digestion.

d. Transport vesicles occasionally bud from the trans Golginetwork to fuse with the

plasma membrane in a process called constitutive exocytosis.

e. Most components of the endocytic vesicle membrane eventuallyare returned to the

plasma membrane for reuse.

6. Match the following organelles with their function:

Organelle Bank:

a. Mitochondria

b. Endoplasmic reticulum

c. Nucleus

d. Lysosome

e. Endosome

f. Peroxisome

g. Golgi apparatus

_____ quality control of mRNA

_____ location of oxidative phosphorylation

_____ responsible for detoxifying organic molecules (livercells)

_____ houses and protects genetic material

_____ responsible for modification and sorting of proteins andlipids

_____ location of ATP synthesis

_____ responsible for sorting endocytosed materials

_____ site of degradation and digestion

_____ responsible for oxidizing toxic molecules

_____ location of lipid synthesis

_____ location of hormone synthesis (adrenal cells)

B. Which organelle(s) from the above list communicate(s) withother organelle(s)

through the use of vesicular transport? ________________

C. Which organelle(s) from the above list receive proteins madein the

cytosol?___________________

D. Through which organelle(s) must proteins pass to reach theorganelle(s) which do not

receive proteins made exclusively in thecytosol?______________

E. Entrance into which organelle(s) requires that proteinsunfold and snake through the

membrane? _________________

F. How do organelles maintain their position in the cytosol? Bespecific.

G. Give 2 advantages that eukaryotic cells gain by havingorganelles.

7. Which of the following statements are true regardingactin?

a. They are arranged in a

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BIOL-4610 Lecture Notes - Lecture 15: Nuclear Pore, Nuclear Localization Sequence, In Situ Hybridization

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