GEN-3000 Lecture Notes - Lecture 10: Dna Replication, Nucleosome, Dna Supercoil
Document Summary
Three models for dna replication: conservative replication. Like a copier: keep original and have a copy: dispersive replication. Old strand is copied and have new strand but the old stand is dispersed into new strand: semiconservation replication. One strand stays in tack: but when old stand splits it acts as a template and have one completely old stand and one completely new in double stranded dna molecule. Grew bacteria and gave 15n as the only nitrogen source. They they isolated the dna and spinner it out: heavy 15n at the bottom. Next 14n medium: dna into dna which is lighter. Spun it down and got a hybrid molecule. If conservative we would have maintained old double stranded dna and the new would be above. After one round there would be a hybrid but after another round it would move closer to. What is seen in most bacterial organisms because of the circular chromosome.
