BIOLCHEM 415 Lecture Notes - Lecture 28: Phosphoenolpyruvic Acid, Aldehyde, Methyl Group

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21 Jul 2016

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Nitrogen is a key part to amino acids. Sustainable amount of nitrogen gas in the atmosphere but unusable because of the triple bond that exists between the two nitrogen. We will first discuss how some bacteria will fix nitrogen into ammonia. Then we will discuss how amino acids are synthesized; firstly by how the nitrogen is captured and then the carbon skeletons and where they come from and how they can be converted to amino acids. We will not cover every 20 amino acids synthesis. Amino acids can be used as precursors to synthesize other molecules. Nitrogen fixation is the process where the atmosphere gas is reduced to ammonia. You need about 225kcal/mol of energy to break the bond and make ammonia. An industrial process, called haber-bosch process, can do nitrogen fixation but required very high temperature and pressure and a catalyst. This is used today but to fix nitrogen into fertilizer.

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Microbiology Unit 2 Applied and Environmental Assignment Part 1:

Identify Nutritional requirements from Chapter 6 & 14 (and review from chapters 1 & 2)

1. Determine which of the 4 macromolecules require the following elements:

a. Carbon: carbohydrates___ lipids___ proteins___ nucleic acids___

b. Nitrogen: carbohydrates___ lipids___ proteins___ nucleic acids___

c. Sulfur: carbohydrates___ lipids___ proteins___ nucleic acids___

2. The essential elements mentioned above are cycled through cells and the earth in biogeochemical cycles like the carbon cycle, the nitrogen cycle, and the sulfur cycle. Select the correct answer and fill in the blanks to summarize these cycles:

a. Carbon dioxide is taken in from the atmosphere by [autotrophs/heterotrophs] and converted to glucose through carbon ________________. When heterotrophs eat autotrophs they catabolize glucose to make ATP through __________________ and waste carbon is released as ___________.

b. There is plenty of nitrogen available in the atmosphere but it is not accessible to organisms because it is in the form of Nitrogen gas (N2). N2 is fixed by [bacteria/plants/animals] and converted to ammonia (NH3.) NH3 can be used by [bacteria/plants/animals] to build their cells. Next, by consuming food with amino acids, [bacteria/plants/animals] can get access to nitrogen for their own cellular needs.

c. There is also a good quantity of sulfur in dirt, rocks, and the atmosphere. Sulfur cycles between H2S => S0 => SO4 => SH groups in Proteins => back to H2S. Using _________________ respiration, anoxygenic photosynthesis, and chemolithoautotrophy [bacteria/plants/animals] convert H2S and S0 to SO4 which is taken in by [bacteria/plants/animals] and used to build their cells. When [bacteria/plants/animals] eat plants they take in essential amino acids containing sulfur and recycle these to make their own proteins.

d. _________________ break down waste and dead organisms and recycle nutrients back into the cycles.

*The C and N are summarized here: https://youtu.be/2D7hZpIYlCA and https://youtu.be/leHy-Y_8nRs

3. Identify the main metabolic classifications:

A microbe that makes its own food by taking carbon from CO₂ in the atmosphere and its energy from sunlight
A microbe that makes its own food by taking carbon from CO₂ in the atmosphere and its energy from reduced inorganic chemicals
A microbe that eats other organisms to get organic carbon and energy
A microbe that uses organic carbon from others but energy from sunlight

Please help me with my microbiology assignment.

orchidherring991

Microbiology Unit 2 Applied and Environmental Assignment Part 1: Identify Nutritional requirements from Chapter 6&14 (and review from chapters 1 & 2) 1. Determine which of the 4 macromolecules require the following elements: a. Carbon: carbohydrates___ lipids___ proteins___ nucleic acids___ b. Nitrogen: carbohydrates___ lipids___ proteins___ nucleic acids___ c. Sulfur: carbohydrates___ lipids___ proteins___ nucleic acids___ 2. The essential elements mentioned above are cycled through cells and the earth in biogeochemical cycles (the carbon, nitrogen, and sulfur cycles.)Select the correct answer or fill in the blanks to summarize these cycles: a. Carbon dioxide is taken in from the atmosphere by [autotrophs/heterotrophs] and converted to glucose through carbon ________________. When heterotrophs eat autotrophs they use catabolism to break down glucose to make ATP and waste carbon is released as ___________. b. There is plenty of nitrogen available in the atmosphere but it is not accessible to organisms because it is in the form of Nitrogen gas (N2). N2 is fixed by [bacteria/plants/animals] and converted to ammonia (NH3.) NH3 can be used by [bacteria/plants/animals] to build their cells. Next, by consuming food with amino acids,[bacteria/plants/animals] can get access to nitrogen for their own cellular needs. c. There is also a good quantity of sulfur in dirt, rocks, and the atmosphere. Sulfur cycles between H2S => S0 => SO4 => SH groups in Proteins => back to H2S.Using _________________ respiration, anoxygenic photosynthesis, and chemolithoautotrophy [bacteria/plants/animals]convert H2S and S0 to SO4 which is taken in by [bacteria/plants/animals] and used to build their cells. When [bacteria/plants/animals]eat plants they take in essential amino acids containing sulfur and recycle these to make their proteins. d. _________________ break down waste and dead organisms and recycle nutrients back into the cycles. *The C and N are summarized here: https://youtu.be/2D7hZpIYlCA and https://youtu.be/leHy-Y_8nRs 3. Match the main metabolic classifications to the description: Classifications: photoautotroph/ photoheterotroph/ chemoautotroph/ chemoheterotroph o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from sunlight o A microbe that makes its own food by taking carbon from CO2 in the atmosphere and its energy from reduced inorganic chemicals o A microbe that eats other organisms to get organic carbon and energy o A microbe that eats other organisms to get organic carbon but gets energy from sunlight 4. Identify the phases of the bacterial growth curve and define what occurs in each phase Phase A = ______________ Definition: Phase B = ______________ Definition: Phase C = ______________ Definition: Phase D = ______________ Definition: Part 2: Unique ecosystems containing microbes 5. Watch the video that shows hydrothermal vents: https://youtu.be/RO3nO98gH_M a. Describe bacteria that live at the vents using terms from the unit 2 reading guide: ________________________________________________________________________ b. Write a 2-3 sentence reflection about hydrothermal vents and the contribution of microbes to this ecosystem: 6. Apply what you have learned to the TED-Ed video about Biofilms: https://youtu.be/pHLP5CZMnL4 a. Give the terms for cell structures mentioned in the video that we learned in Chapter 4 and 7: i. âsticky appendagesâ thatallows bacteria to attach and begin the biofilm: _______________ ii. Extracellular matrix/gooey matrix that holds cells together: ____________________________ iii. Exchange genetic information while packed together: ____________________________ iv. Communicate with each other by _________________________________________ sensing. v. Produce chemical to kill microbial competitors: ____________________________ factor.

After working through the macromolecules in your friendâs extremely pale burrito, you look at her and think you might have an idea about what is going on. You ask Maria, âDo you usually eat like this?â âYup,â she says, âI love all the carbs, rice, and bread, as well as cheese. Sometimes I eat a piece of fruit or some carrot sticks. And energy drinks!â You then point out to your friend that she seems to be getting very little protein, a major class of macromolecules. But she is getting plenty of carbohydrates and lipids in the form of animal fats.

1. While your body can convert the monomers of macromolecules from one to the other, it has to have the raw materials in the form of the appropriate atoms. You explain to your friend that when she eats proteins, they are broken down into amino acids through the process of INSERT ANSWER and can then be rebuilt into new proteins through the process of INSERT ANSWER.

a. If your body does not take in protein, but only carbohydrates and lipids, what atoms will it be missing? Hint: There are two, and you can figure this out by comparing the structures of the monomers for proteins and carbohydrates as well as the basic structure of lipids. INSERT ANSWER

b. For the above atoms, how many electrons are in the outer shell? INSERT ANSWER

c. For the above atoms, what type of bond will they make within the amino acid monomer? INSERT ANSWER

d. Even if your friend does obtain the correct atoms, there are still a couple amino acids that your body cannot manufacture from scratch, but must be ingested. What are these amino acids? INSERT ANSWER

e.Why might a lack of protein or the wrong types of proteins lead to your friendâs fatigue? INSERT ANSWER

BIOLCHEM 415 Lecture Notes - Lecture 28: Phosphoenolpyruvic Acid, Aldehyde, Methyl Group

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