HSS 1100 Lecture 3: 3 Diagnostic microbiology

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27 Jun 2022
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HSS 1100 Full Course Notes
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HSS 1100 Full Course Notes
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Laboratory procedures to find out what pathogen is causing symptoms in patient (helps treat patient appropriately) Done with isolated pure culture through a. Separate bacteria from other bacteria that could mess with it. Use inoculation loop to spread sample on plate to grow bacteria from single cell to a colony. Idea is to thin out bacteria that could be in a sample. After incubation, different colonies should be present. Are used for enumeration of bacteria (to count bacteria cells) Not expecting to see floral bacteria, so if anything grows it"s probably pathogens. To keep bacteria alive for further testing. Can also use freezers that can reach below - Looking for clues of a pathogen with any information to eliminate all suspects in order to get actual guilty bacteria. Can look at colony morphology (shape) or remove part of the colony to look at the cellular morphology (with microscope)

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