A plasmid that carries two antibiotic resistance genes, to ampicillin and tetracycline, respectively, has one restriction site for EcoRI located within the tetracycline resistance gene. Eukaryotic DNA containing a gene of interest (g.o.i.) has been digested with diffferent restriction enzymes, none of which cut within the gene of interest sequence). The resultant fragments are added to plasmid DNA that has been digested with EcoR1 only; the mixture was then incubated with DNA ligase. Resulting plasmids (recombinant and otherwise) are added to competent bacteria lacking resistance genes and growing in nutrient broth. The bacteria are then plated and allowed to grow overnight producing isolated colonies. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin; nutrient broth plus tetracycline; nutrient broth plus ampicillin and tetracycline; and nutrient broth containing no antibiotics.
1. The bacteria containing the plasmid with the gene of interest successfully insertedin would grow in
A. Tthe nutrient broth only.
B. The nutrient broth, the ampicillin broth, and the tetracycline broth.
C. Tthe nutrient broth and the tetracycline broth only.
D. The ampicillin broth and the nutrient broth.E.Tthe ampicillin and tetracycline broth only.
2. Bacteria transformed with the plasmid alone (not containing any eukaryotic DNA), would grow
A. Only in the broth containing both antibiotics.
B. In the nutrient broth plus ampicillin, but not in the broth containing tetracycline.
C. In the broth containing tetracycline, but not in the broth containing ampicillin.
D. Only in the broth that contained no antibiotics.
E. In all four types of broth.
4. Bacteria that did not take up any plasmids at all would grow in which media?
A. The tetracycline and ampicillin broth only
B. The nutrient broth and the tetracycline broth only
C. The nutrient broth and the ampicillin broth only
D. The nutrient broth only
E. All four broths
A plasmid that carries two antibiotic resistance genes, to ampicillin and tetracycline, respectively, has one restriction site for EcoRI located within the tetracycline resistance gene. Eukaryotic DNA containing a gene of interest (g.o.i.) has been digested with diffferent restriction enzymes, none of which cut within the gene of interest sequence). The resultant fragments are added to plasmid DNA that has been digested with EcoR1 only; the mixture was then incubated with DNA ligase. Resulting plasmids (recombinant and otherwise) are added to competent bacteria lacking resistance genes and growing in nutrient broth. The bacteria are then plated and allowed to grow overnight producing isolated colonies. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin; nutrient broth plus tetracycline; nutrient broth plus ampicillin and tetracycline; and nutrient broth containing no antibiotics.
1. The bacteria containing the plasmid with the gene of interest successfully insertedin would grow in
A. Tthe nutrient broth only.
B. The nutrient broth, the ampicillin broth, and the tetracycline broth.
C. Tthe nutrient broth and the tetracycline broth only.
D. The ampicillin broth and the nutrient broth.E.Tthe ampicillin and tetracycline broth only.
2. Bacteria transformed with the plasmid alone (not containing any eukaryotic DNA), would grow
A. Only in the broth containing both antibiotics.
B. In the nutrient broth plus ampicillin, but not in the broth containing tetracycline.
C. In the broth containing tetracycline, but not in the broth containing ampicillin.
D. Only in the broth that contained no antibiotics.
E. In all four types of broth.
4. Bacteria that did not take up any plasmids at all would grow in which media?
A. The tetracycline and ampicillin broth only
B. The nutrient broth and the tetracycline broth only
C. The nutrient broth and the ampicillin broth only
D. The nutrient broth only
E. All four broths
