I need help with these questions about Genetics Laboratory techniques involving DNA extraction please:
1.) After precipitation of DNA and centrifugation at maximum speed, supernatant is discarded and ethanol is added to the remainder to wash the pellet.
a. True
b. False
2.) ____________ precipitation of DNA and centrifugation at maximum speed and washing the pellet using ethanol, _________________ is added to the pellet to elute the DNA in the solution.
a. After - DNA rehydration buffer
b. Before â isopropanol
c. After â lysis buffer
d. Before â ethanol
3.) Using a heat block after adding DNA rehydration buffer to the pellet will accelerate the process of elution.
a. True
b. False
4.) In an electrophoresis gel, DNA moves from the negative electrode towards the positive electrode because: (1) the molecule of DNA is negatively charged and (2) the loading dye moves from the positive electrode towards the negative electrode.
a. True
b. False
I need help with these questions about Genetics Laboratory techniques involving DNA extraction please:
1.) After precipitation of DNA and centrifugation at maximum speed, supernatant is discarded and ethanol is added to the remainder to wash the pellet.
a. True
b. False
2.) ____________ precipitation of DNA and centrifugation at maximum speed and washing the pellet using ethanol, _________________ is added to the pellet to elute the DNA in the solution.
a. After - DNA rehydration buffer
b. Before â isopropanol
c. After â lysis buffer
d. Before â ethanol
3.) Using a heat block after adding DNA rehydration buffer to the pellet will accelerate the process of elution.
a. True
b. False
4.) In an electrophoresis gel, DNA moves from the negative electrode towards the positive electrode because: (1) the molecule of DNA is negatively charged and (2) the loading dye moves from the positive electrode towards the negative electrode.
a. True
b. False