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2. Which of the following would the MSA medium NOT be considered: Select one:

a. Complex medium

b. Chemically defined medium

c. Differential medium

d. Selective medium

3. If you took a swab from a patient and cultured it on a MSA plate, which of the following could be identified by just using this test? Select one:

a. Staphylococcus epidermidis

b. Escherichia coli

c. Staphylococcus aureus

d. Micrococcus luteus

4. If you streaked the 3 organisms in this lab on a PEA plate and after incubating for 2 days at 37 degrees Celcius nothing grew (but they did grow on a NA plate made at the same time and grown in the same conditions), which of the following is the most likely cause for this result? Select one:

a. The agar was toxic.

b. The beef extract was bad.

c. The temperature was too low.

d. Too much phenylethyl alcohol was used

. e. The temperature was too high.

5. Why is the NA plate inoculated in addition to the Columbia CNA with 5% sheep blood agar plate? Select one:

a. Used to compare growth on non-selective medium

b. Used as second selective medium

c. Used to promote hemolysis

d. Used because it is easier to see

e. Used as a practice plate

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Related questions

1. The pH for the water in the MSA recipe is expected to be from 7.2-7.6. If the pH was too low, what could you expect to see? Select one:

a. The entire plate would be yellow

b. Nothing abnormal

c. The agar would crack at pH 6.5

d. E. coli would turn purple

2. Which of the following would the MSA medium NOT be considered: Select one:

a. Complex medium

b. Chemically defined medium

c. Differential medium

d. Selective medium

3. If you took a swab from a patient and cultured it on a MSA plate, which of the following could be identified by just using this test? Select one:

a. Staphylococcus epidermidis

b. Escherichia coli

c. Staphylococcus aureus

d. Micrococcus luteus

4. If you streaked the 3 organisms in this lab on a PEA plate and after incubating for 2 days at 37 degrees Celcius nothing grew (but they did grow on a NA plate made at the same time and grown in the same conditions), which of the following is the most likely cause for this result? Select one:

a. The agar was toxic.

b. The beef extract was bad.

c. The temperature was too low.

d. Too much phenylethyl alcohol was used

. e. The temperature was too high.

5. Why is the NA plate inoculated in addition to the Columbia CNA with 5% sheep blood agar plate? Select one:

a. Used to compare growth on non-selective medium

b. Used as second selective medium

c. Used to promote hemolysis

d. Used because it is easier to see

e. Used as a practice plate

1.

If you were to make Columbia CNA with 5% sheep blood agar plates with twice as much colistin, how would the results likely be affected?

Select one:

a. Less Gram-positive growth, more Gram-negative growth

b. Less Gram-positive growth, no or even less Gram-negative growth

c. More Gram-positive growth, more Gram-negative growth

d. More Gram-positive growth, no or even less Gram-negative growth

e. Same Gram-positive growth, no or even less Gram-negative growth

2.

In Mannitol Salt Agar lab, you were told to invert the plate before incubation. This is a common practice to avoid:

Select one:

a. Condensation drops

b. Increased smell

c. Decrease oxygen content

d. Bacteria from growing in wrong direction

e. Airborne contamination

3.

If you see a yellow halo surrounding a colony on a MSA plate, which of the following is likely to be true?

Select one:

a. Glycolysis occurred

b. The organism is Staphylcoccus epidermidis

c. Colonies are not isolated

d. Fermentation occurred

e. Colony is Gram-negative

4.

Which one of the following is found in MSA plates, but not in NA plates?

Select one:

a. Phenol red

b. Agar

c. Tryptose

d. Peptone

e. Beef extract

5.

Why are the three organisms of Phenylethyl Alcohol Agar lab inoculated on the same plate?

Select one:

a. To avoid contamination

b. For easy viewing purposes.

c. To save resources

d. To avoid fluctuations in medium content and environmental effects.

6.

For which reason would you most likely use a PEA plate for culturing after taking a sample from a patient?

Select one:

a. To isolate Streptococcus and Staphylococcus from other fecal bacteria.

b. To detect Escherichia contamination on the skin.

c. To find E. coli strains associated with food poisoning.

d. To identify gram-negative anaerobes in the patient's flora.

The epiglottis acts as a

voice box.

filter for microorganisms and debris.

cover over the larynx.

producer of mucus.

ANSWER:

(Level 1, Objective 1)

Collectively, the nose, oral cavity, pharynx, epiglottis, and larynx make up the

nasopharynx.

oropharynx.

laryngopharynx.

upper respiratory system.

ANSWER:

(Level 1, Objective 1)

The cilia present on cells of the lower respiratory system function by:

trapping microorganisms and foreign debris.

propelling microorganisms and particles out.

preventing colonization by potential pathogens.

all of the above.

ANSWER:

(Level 1, Objective 2)

The most common causative agent of a swollen and painful oropharynx is

Streptococcus pyogenes.

Arcanobacterium haemolyticum.

Corynebacterium diphtheriae.

Rhinovirus.

ANSWER:

(Level 1, Objective 3)

Infections associated with cystic fibrosis are due to inflammation of the:

bronchi.

bronchioles.

lungs.

alveoli.

ANSWER:

(Level 1, Objective 3)

A physician sends a nasal washing for viral culture. This specimen is

unacceptable because it is contaminated with upper respiratory flora.

acceptable as long as it contains epithelial cells.

unacceptable if it is for the detection of influenza or respiratory syncytial viruses.

acceptable if it has a thick consistency and is yellow or green in color.

ANSWER:

(Level 2, Objective 4)

A patient is unable to produce adequate sputum for routine, acid-fast, and fungal cultures. The physician may attempt to obtain a/an

induced sputum.

transtracheal aspirate.

bronchoalveolar lavage.

pleural fluid.

ANSWER:

(Level 1, Objective 4)

9. Two throat swabs are received from a 6-year-old female with orders for a rapid screen for group A Streptococcus. One swab is tested using the group A Streptococcus rapid screen, and it is negative. The second swab is used to inoculate

a. sheep blood agar.

b. chocolate agar.

c. no culture is necessary.

d. a and b.

ANSWER:

(Level 1, Objective 6)

Which of the following disease states requires the addition of selective and/or differential medium to ensure recovery of potential pathogens from the respiratory system?

Pertussis

Cystic fibrosis

Laryngitis

a and b

all of the above

ANSWER:

(Level 2, Objective 6)

11. A throat culture provides the following results:

Moderate growth of alpha-hemolytic, catalase-negative, optochin-susceptible, gram-positive diplococci

Heavy growth of alpha-hemolytic, catalase-negative, optochin-resistant, gram-positive cocci

Heavy growth of gamma-hemolytic, oxidase-positive, glucose- and maltose­-positive, gram­ diplococci

You would report

moderate growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Neisseria meningitidis and heavy growth of normal upper respiratory flora.

heavy growth of normal upper respiratory flora.

ANSWER:

(Level 3, Objective 7)

Yeast colonies in a sputum culture are considered significant when they

are present.

comprise 25% of the organisms present.

comprise 50% of the organisms present.

comprise 75% of the organisms present.

ANSWER:

(Level 1, Objective 7)

13. An 8-year-old male presents with a red and sore throat. The rapid group A Streptococcus screen is negative, and a sheep blood agar is inoculated. At 24 hours, there are no beta-hemolytic colonies present and moderate growth of normal upper respiratory flora. At 48 hours, the same culture results are present. Which of the following statements does not explain these results?

a. His sore throat is due to Haemophilus influenzae, and it will not grow on blood agar.

b. The culture was taken very early in the infection.

c. The patient received treatment prior to specimen collection.

d. The causative agent is not bacterial.

ANSWER:

(Level 2, Objective 8)

17. A quantitative bronchial alveolar lavage culture requires:

1. sheep blood agar.

2. chocolate agar.

3. 0.01 mL pipette.

4. 0.001 mL pipette.

a. 1 and 3

b. 1, 3, and 4

c. 2, 3, and 4

d. 2 and 4

ANSWER:

(Level 1, Objective 9)

18. A bronchial washing is plated to a BCYE. This agar should be examined under a laminar flow hood due to the potential for recovery of Legionella pneumophila and

a. Bordetella pertussis.

Francisella tularensis.

mould.

Pneumocystis jiroveci.

ANSWER:

(Level 2, Objective 9)

20. A physician submits a sputum with a culture request for Legionella. Select the growth medium that should be used to cultivate this organism.

a. 7H10

b. TCBS

c. BCSA

d. BCYE

ANSWER:

(Level 1, Objective 6)

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