Question

1. Choose the TWO correct statements describing the citrate cycle.

	ATP is an indirect product of the citric acid cycle
	Acetyl CoA can increase the pool of citric acid cycle intermediates
	Fatty acids can increase the pool of citric acid cycle intermediates
	ADP inhibits α-ketoglutarate dehydrogenase
	The pool of mitochondrial oxaloacetate can be increased by pyruvate
	All enzymes of the citric acid cycle are found soluble and free in the mitochondrial matrix

2.Which molecule in the net reaction of the citrate cycle contributes to the inhibition of pyruvate dehydrogenase?

	NADH
	H+
	H2O
	FAD

3. How would a high NADH to NAD⁺ ratio be expected to affect the pyruvate dehydrogenase reaction?

	The E1 subunit is phosphorylated by pyruvate dehydrogenase kinase, and the catalytic activity of pyruvate dehydrogenase decreases.
	The last step of the pyruvate dehydrogenase reaction is blocked, resulting in a decrease in activity.
	The pyruvate dehydrogenase phosphatase-1 enzyme would increase, resulting in pyruvate dehydrogenase activation at an accelerated rate.
	The pyruvate dehydrogenase kinase enzyme activity would increase, resulting in an increase in pyruvate dehydrogenase activity.