BIOL 301 Study Guide - Midterm Guide: Point Mutation, Cloning, Restriction Enzyme
10 Apr 2018
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Molecular markers for precise gene mapping determine recombinant frequency with different markers -cross with mapping line, first determining chromosome, then determining location on chromosome. Pcr for marker not mutated gene -for diploid, must look at progeny. Create heterozygote for linked genes, then cross to tester line (homo recessive) Slow, many crosses, must wait until progeny is older to see morphological differences. Have to use tester stocks (mapping lines) -repeat. Can"t discriminate heterozygotes from wild-type: testing molecular markers in the two parental ecotypes/strains as well as an. Identify small # of candidate genes that could be mutated mutant to mapping line. Vntrs slower migration with more repeats (heterozygote has two bands) Caps snps that alter (add or remove) a restriction enzyme site (heterozygotes have 3 bands. Sslps: 8-30bp polymorphisms between ecotypes due to copy number. To get precise number for lower rfs, must test more individuals to protect from random event.
