BIO230H1 Study Guide - Midterm Guide: Two-Dimensional Gel Electrophoresis, Isoelectric Point, Sigma Factor

72 views5 pages

azurefrog421

10 Feb 2018

School

Department

Course

Professor

For unlimited access to Study Guides, a Grade+ subscription is required.

avalack09 and 39882 others unlocked

BIO230H1 Full Course Notes

Verified Note

42 documents

Document Summary

Product of genome expression is transcriptome, which is maintained by transcription. The rna molecules present in a cell at a particular time. Second product of genome expression is the proteome, maintained by translation. Colle(cid:272)tio(cid:374) of p(cid:396)otei(cid:374)s i(cid:374) a (cid:272)ell that defi(cid:374)e the (cid:272)ell"s fu(cid:374)(cid:272)tio(cid:374)s. Proteins; break down sugars, carry energy, carry out cell processes. 2d gel electrophoresis: allows separation of dna by weight and isoelectric point. Different types of cells in the same organism contain the same genome. I. e a neuron will contain the same genome as a skin cell, so the difference in the cells is equated to the difference in genome expression of each cell. At a single time only 30-60% of the 25000 genes are expressed in the human genome. I. e. hemoglobin is only expressed in your rbc, therefore there is different levels of genome expression across cell types. Genome expression is regulated through the different steps in dna to protein synthesis.

Related Questions

Cell Biology (#1-5)

1) What does a non-synonymous DNA mutation cause?

a) No amino acid change

b) A change in the reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

2) What does a non-sense DNA mutation cause?

a) No amino acid change b) Alters the normal reading frame of the mRNA

c) The substitution of an amino acid

d) Creates a premature termination codon

e) All of the above

3) If you sequence the genome of 100 cells from different tissues of the same multicellular organism. How do you expect the sequences to be?

a) 10% identical c)100% identical

b) 50% identical d) 100% different e) will depend on the tissue selected

4) Multicellular organisms are comprised of a variety of cells with different morphologies and different functions. Which is the underlying mechanism that leads to the generation of diverse cell in one organism?

a) The expression of all genes in all cell types

b) The duplication of the genome in certain cell types

c) The elimination of specific group of genes from different cell types

d) The differential expression of particular sets of genes requires for a particular cellular phenotype

e) All of the above

5)You are growing bacteria which can use lactose and glucose. The culture medium has lactose, which induces transcription of the enzymes required to use lactose. If you add glucose to the medium. What do you expect to happen?

a) The bacteria will keep using lactose

b) The bacteria will shut down the Lac operon and use glucose

c) The bacteria will stop growing

d) The bacteria will die

e) The bacteria will use both lactose and glucose

salmoneagle868

1. Which cellular processes occur as a consequence of differential control of gene expression in eukaryotic cells?

a) Cell Differentiation

b) Differential Translation

c) Differential Polymerization

d) Differential DNA Replication

e) Differential tRNA Degradation

2) Multicellular organisms are comprised of a variety of cells with different morphologies and different functions. Which is the underlying mechanism that leads to the generation of diverse cell in one organism?

a) The expression of all genes in all cell types.

b) The duplication of the genome in certain cell types.

c) The elimination of specific group of genes from different cell types.

d) The differential expression of particular sets of genes required for a particular cellular phenotype

e) All of the above

3) Which of these statements defines paracrine cell signaling?

a)		A messenger molecule released into the blood stream to bind to a receptor on a cell far away from the release site.
b)		A messenger molecule released by a cell into the extracellular space that binds to receptors on the surface of the same cell.
c)		A messenger molecules released by a cell into the extracellular space that travels short distances to bind to a receptor in a nearby cell.
d)		A messenger molecule release by a tissue into the lumen of an organ that diffuse to bind to receptors far away from the release site.
e)		All of the above.

4) Which effect has the binding of the ligand to a G-protein coupled receptor on intracellular G-protein?

a)		ATP is hydrolyzed, activating phosphorylation
b)		GTP is exchanged for GDP on the G protein, inactivating the G protein
c)		GDP is exchanged for GTP on the G protein, activating the G protein
d)		GMP is released from the G-protein, inactivating the receptor
e)		None of the above

coffeehippopotamus191

1) A valuable tool for assessing the transcriptome (i.e., thegenes actively expressed in cells or tissues in response tospecific signals or environmental changes) is
a) DNA microarrays or DNA chips.
B) SNP analysis.
C) protein arrays.
D) All of the choices listed are correct.

2) Why is the eukaryotic proteome so much larger than the numberof genes found in an organism?

A) Proteins are large molecules.

B) Differential intron splicing can produce more than one proteinproduct from a gene, and proteins are further posttranslationallymodified.

C) Many proteins are made from mitochondrial DNA, and this is nottaken into account.

D) The statement is incorrect: the proteome is equal to the numberof genes.

3)The number of mRNA transcripts in a cell fluctuate over timeas the pattern of gene expression changes. These transcripts arecollectively referred to as

A)regulatory transcripts. B)the transcriptome. C)theexpresstome. D)the proteome.

pearjackal397

BIO230H1 Study Guide - Midterm Guide: Two-Dimensional Gel Electrophoresis, Isoelectric Point, Sigma Factor

BIO230H1 Full Course Notes

Document Summary

Get access

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Biology

Concepts of Biology

Essentials of Biology

Related Documents

BIO230H1 Chapter Notes - Chapter 1-7: Two-Dimensional Gel Electrophoresis, Essential Amino Acid, Sigma Factor

BIO230H1 Lecture Notes - Lecture 2: Two-Dimensional Gel Electrophoresis, Dna Microarray, Transcriptome

BIO230H1 Lecture Notes - Lecture 3: Multicellular Organism, Cyclic Adenosine Monophosphate, Enzyme

Related Questions