BIOL3040 Study Guide - Quiz Guide: Electron Microscope, Protein Folding, Microscopy
Document Summary
Light microscopy- brightfield illumination, phase contrast, dic, fluorescence. Limit of resolution/diffraction limit- the limit in the ability to see 2 neighboring points in a field as distinct entities (about 200nm for light microscopy) (depends on wavelength and numerical aperture) Scanning em- electrons reflect off cell, 3d images. Model organism/model system- any organism used as a model for understanding how certain cells and systems work. Cell culture/tissue culture- culture of cells taken from living things and grown in lab. Cells require presence of growth factors in the medium to proliferate(multiple) in vitro (outside normal biological context) Clonal/line- grown up from a single cell. Immortalized- lost the normal limit to replicative capacity. Co-culture- two (or more) different cell types grown together. Proteins- building blocks for structure and enzymes (enzymes, motors, molecular machines, structural components, transporters, signaling components). Polypeptide- (a protein) is made from chain of amino acids. Side chains- confer functionality to proteins (charge and polarity)
