BIOL 1020 Chapter Notes -Homeotic Selector Gene, Gene Targeting, Transgene

18 views3 pages

mauvesnake886

25 Nov 2014

School

Department

Course

Professor

For unlimited access to Textbook Notes, a Class+ subscription is required.

Document Summary

Homologous recombination- two homologous dna sequences interact and undergo an exchange of genetic information. If the arms are homologous to the endogenous gene sequence, there is a chance where the two regions can undergo homologous recombination (x marks in the slide) You will end up with endogenous locus with the inserted gene that you wanted. You need a promoter to drive the expression of the transgene. Blue is target region: targets to right region (a, vector gets into nucleus but inserts into the wrong region ( pink) (b, target vector gets into the nucleus but doesn"t do anything ( no change in the genome. This will have an effect on the resistance of that cell. The cells that have yellow have vectors that have been inserted into the genome so they have antibiotic resistant gene (neomycin). The third possibility has no change in the genome so that will not be resistant to antibiotics (blue).

Related Questions

Flies with a dominant mutation in a particular gene have an additional set of wings. Through mapping techniques, you identify a candidate gene, gene X, on an autosomal chromosome that you think results in extra wings when mutated. When you sequence gene X in the mutant fly with extra wings, you notice a missense mutation in the middle of the coding region of gene X. What is the best way to verify that the mutation in gene X is responsible for the dominant phenotype?

a. Starting with a wild-type fly, knockout gene X to generate a null allele and see if flies that are heterozygous or homozygous for the null allele have extra wings.

b. Starting with the mutant fly that is heterozygous for the dominant allele, insert a wild type copy of gene X (complete with its own promoter) elsewhere in the genome of the fly and see if it rescues the mutant and results in normal/wild-type wings.

c. Starting with a mutant fly that is heterozygous for the dominant allele, replace the dominant mutant allele with a wild type version of the gene via homologous recombination and see whether flies with the replacement have normal/wild type wings.

d. Starting with a homozygous wild-type fly, replace one copy of wild-type gene X with a version carrying the dominant mutation via homologous recombination, and see whether flies with the replacement have extra wings.

e. either C or D

You have a strain of flies that are homozygous for a deletion of 2 neighboring genes, A and B (genotype aabb). Wild type flies have transparent wings but these aabb flies have rainbow-colored wings. Which of the following experiments would provide you with more information about which of these genes (if either) is responsible for the rainbow-colored wings?

a. Starting with a wild-type fly, create a transgenic fly that is homozygous for deletion in both genes A and B and see if you get the rainbow-colored wings.

b. Delete gene A alone and gene B alone in 2 independent wild-type flies and see whether flies homozygous for either deletion have rainbow-colored wings.

c. Starting with a mutant fly with rainbow-colored wings, introduce both wildtype genes A and B into a new place in the genome of the fly and see if normal wing color is restored.

d. non of the above

Q1.

You are a genetic engineering hired by the biotechnology company, Genes 'R' Us, to use genetic engineering to develop a new rose that glows. Place the following steps in the order you would need to perform them to successfully genetically engineer the rose with this trait.

-Extract all of the DNA from a firefly cell

-Use traditional plant breeding techniques to improve the performance of the rose, which has the new glowing gene (transgene)

-Clone the single gene that encodes the glowing protein

-Insert the glowing gene into single rose plant cell using either the gene gun or agrobacterium

-Modify the glowing gene so it acts In the way you want it once its inside the rose plants

-Identify the firefly as an organism that would have a gene encoding a protein for a âglowing traitâ

Q2.

Genetic engineering is different from traditional plant breeding in that (select all that apply)

___genetic engineering 'physically' removes DNA (genes) from one organism and inserts them into another

___plant breeding and classical biotechnology relies on natural sexual reproduction to create variation while genetic engineering introduces variation one or a few genes at a time into a single cell.

___genetic variation used in classical biotechnology (plant breeding) comes from mutations in genes already found in that crop species. In genetic engineering, new genes are added to the genetic makeup of a plant

___genetic engineers can move DNA from any organism into any organism, even if they are not in the same species.

Q3.

Which method of plant transformation works and depends upon an organism that is a "naturally occurring" genetic engineer?

A)Gene Gun

B)Agrobacterium

C)Microneedle insertion into a plant egg cell

D)Electric Shock of Plant Cells

rubydeer239

Q.In the non-9:3:3:1 ratio dihybrid cross below, there are 3 classes of phenotypes: white, pink, red which of the following is NOT true?

9 A_B_ white

3 A_bb pink

3 aaB_ red

1 aabb red

a.) phenotype is dependent on the genotype of gene B

b.) This is an example of recessive episasis

c.) aa is epistatic to B_, and bb

d.) phenotype is dependent on the genotype of gene A

Q,When choosing a selectable marker, you...

a.) choose a enhancer for your gene of interest

b.) choose a rescue gene to save your gene of interest

c.) choose a resistant gene that will allow for colony screening

d.) choose a gene that encodes for DNA replication

Q.The following steps detail an experiment resulting in transgenic mice with a knockout gene for fur color. Recall that agouti (brown) mice are A/A;M/M and black fur coats are a/a;M/M . List the steps in their correct chronological order.

1.Inject transformed agouti embryotic cells into an a/a;M/M embryo to produce A/A;M/m and a/a;M/M altered embryo

2.Self-cross hemizygous mice to produce homozygous transgenic mice (A/-;M/m)

3.Create a a targeting vector containing tk+ for ganciclovir sensitivity, neoR for neomycin resistance, and the gene encoding fur color.

4.Observe mice with chimeric phenotype and cross them with black a/a;M/M mice to produce transgenic hemizygous progeny (A/a;M/m)

5.Isolate all transformed embryonic stem cells that show homologous recombination and inject them into a embryonic stem cells of an agouti mouse.

a.) 3,5,4,1,2

b.) 1,5,3,4,2

c.) 3,5,1,4,2

d.) 5,3,2,4,2

If dog 1 has is black with alleles (A_B_) , and dog 2 is brown with (aaB_), and dog 3 is yellow with (_ _bb) alleles, what kind of epistasis is this and what color would dog 4 be if he was (AAbb).

1. Recessive epistasis, Yellow

2. Recessive epistasis, Black

3. Dominant epistasis, Yellow

4. Dominant epistasis, Black

A gene interaction in which pair of recessive genes at one locus prevents expression of dominant allele at another locus called

a. incomplete dominance

b. epistatis

c. complete dominance

d. polygenic inheritance

BIOL 1020 Chapter Notes -Homeotic Selector Gene, Gene Targeting, Transgene

Document Summary

Get access

Related Documents

BIO SCI 97 Lecture 24: Lecture 22 Notes

BIOC14H3 Lecture Notes - Lecture 5: Integrase, Olfactory Bulb, Recognition Sequence

BIOL 202 Lecture Notes - Lecture 10: Vulva, Immunocytochemistry, Genetic Screen

Related Questions