BI346 Lecture Notes - Lecture 1: Helicase, Dna Replication, Central Dogma Of Molecular Biology
Document Summary
The study of the molecular mechanism of cell function at the cellular level, especially replication, transcription and translation. When dna makes more copies of itself = replication. Misconception of transcription: the dna molecule is a template and rna is the complementary base pairing. They are chemically different than dna, they are only complementary. Semi-conservative production of dna one strand (backbone) is from parental and the second strand is synthesized. At the origin (ori) recogonized by the initaor protien" recurits dna helicase. Helicase just unwinds, doesn"t make dna: elongation. Beginning with rna primer, dna polymeriase synthesized in 5" to 3" direction causing leading and lagging strand: termination. In eukaryotes or when two forks meets, or where end of linear chromosomes is reached. Don"t forget, you need a primer to start replication. There is so much genetic material, so you need many positions. Replication fork continuous synthesis = non continuous = opposite direction this is why we get okaski fragments.
