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13. Taking into account your response to question 5 from the group of questions I submitted. When a 10×PBS solution, describe how you would prepare 1liter of 1× working solution PBS, including which glassware you would use.Will you need to adjust the pH again?

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Sixta Kovacek
Sixta KovacekLv2
8 Jan 2018
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Related questions

You have just begun working in a research laboratory. Your advisor asks you to make a series of stock solutions. He also explains that you may use a pH meter to adjust the pH. Calculate how much of the solid reagent you would add to make the following:

500 mL 1 M Tris, pH 8.0 (MW = 121.4 g/mole)

1.0 L 5 M NaCl (MW = 58.44 g/mole)

10 mL 100 mg/mL Ampicillin (MW = 371.4 g/mole)

500 mL 0.5 M EDTA (Ethylene Diamine Tetra-acetic Acid; MW = 292.2 g/mol)

200 mL 1 M MgCl2 (Note: MgCl2 is sold by the chemical company as co- crystallized with H20. Thus, the MW of MgCl2 6H20 is 203.30 g/mole)

For your first experiment, you need to make a solution that is commonly called TE and stands for Tris-EDTA. It is comprised of 10 mM Tris, 1 mM EDTA, pH 8.0. Calculate and describe how you would make 100 ml of TE using the stock solutions that you have already made above in Question 1.

Your advisor asks you to make two concentrated stock solutions for the whole laboratory to use. He explains that it is common to make a concentrated solution to dilute for use. Thus, a 10x solution refers to one that is 10-fold concentrated. Calculate how you would make 1.0 L of each of the following:

A. 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW = 121.4 g/mole

Boric Acid MW = 61.84 g/mole EDTA MW = 292.2 g/mole

B. 50x TAE (2 M Tris, 2 M Acetic acid, 0.5 M EDTA) Tris MW = 121.4 g/mole

Concentrated Acetic acid (glacial) comes as a solution and is 17.4 M EDTA MW = 292.2 g/mole

Your polyacrylamide gel electrophoresis experiment requires 1.0 L of 0.5x TBE. Calculate and describe how you would make this solution using your stock solutions

I have this questions for my homework i need help with them look please it is important i need help so please give me the correct answers for each question and also please be clear to understand and aslo be correct it is very important becuase i need them so i really need the right answers. please help me and on Questions 6 and 9 please help me with it hope you can help me with the correct answers please

Question 5

At a constant temperature, if you increase the number of unsaturated fatty acid chains in the phospholipid, the membrane will become......

less fluid
more fluid
fluidity will be unchanged

Question 6

Bacteria in a hot spring at 80 degrees celsius would be expected to have ______________ in their cell membrane

high proportions of saturated phospholipids
high proportions of unsaturated phospholipids
an equal ratio of saturated to unsaturated phospholipid

Question 7

Membrane proteins have an important role in membrane fluidity

True
False

Question 8

The HIV virus can't attach to cells that have a mutation in the CCR5 gene and so such individuals are resistant to HIV. What population of people have a higher incidence of the mutant CCR5 gene and why?

europeans because their ancestors survived the bubonic plague
North Americans because their ancestors survived small pox
europeans because they have a longer evolutionary past with HIV
Africans because they have a longer evolutionary past with HIV

Question 9

Nematode trapping fungi have ______________ molecules called ______________ that recognize specific carbohydrates on the surface of the nematode worms. This is how a fungus can recognize that its prey, the nematode worm, has wriggled into the loop. Write two words on lowercase separated by a space with no other syntax

Question 10

Diffusion requires energy

True
False

Question 11

The net direction of molecule movement when molecules diffuse is

down their concentration gradient
random directions

Question 12

In osmosis, water molecules from a solution of higher solute concentration cross a semi-permeable membrane into a solution of lower solute concentration?

True
False

Question 13

Which best defines osmosis?

In osmosis, water from a solution of lower solute concentration move across a semi-permeable membrane into a solution of higher solute concentration?
In osmosis, water from a solution of higher solute concentration move across a semi-permeable membrane into a solution of lower solute concentration?

Question 14

Animal cells placed into a hypotonic solution would most likely?

lyse (burst)
shrink
remain unchanged in size

Question 15

Glucose and amino acids move into cells via carrier proteins, the same protein can move glucose or amino acids

True
False

Question 16

ATP powered pumps move substances into and out of cells by diffusion?

True
False

Question 17

For every 3 Na ions moved out, 2 K ions are moved into a cell by one cycle of the sodium-potassium pump.

True
False

Question 18

Bulk transport of fluid and any of the substances within that fluid is called

pinocytosis
phagocytosis
receptor-mediated endocytosis
exocytosis

Please answer all the questions answering only one question is not helpful :

Calculations on Multiple Stock Solutions—be sure to include the amount of water added and include all units

11. Calculate how to use the 1 M Tris and 0.2 M EDTA stock solutions to make the 400 ml of a of 10 mM Tris and 1 mM EDTA solution.

12. Calculate how to use a 3 M NaCl and 10% NP40 stock solutions to make a 500 ml of a 300 mM NaCl and a 0.2% NP40 working solution.

Calculations on Simple Dilutions—be sure to include the amount of water added and include all units

13. Calculate the volumes necessary to make 200 ml of a 1:5 dilution of a sample.

14. Calculate the volumes necessary to make 50 ml of a 1:8 dilution of a sample

15. Calculate how you would dilute a cell suspension containing 3,000,000 cells/ml to obtain 10 ml with 100,000 cells/ml

16. Calculate the volume of a cell suspension containing 800,000 cells/ml you would need to obtain a total of 250,000 cells

Practice Calculations on Serial Dilutions—be sure to include the amount of water added and include all units

17. Calculate and describe (or diagram) how you would use a 10% SDS solution to prepare a final volume of 20 ml of each of the following solutions: 8%, 4%, 2%, 1% 0.5%.

18. Calculate and describe (or diagram) how you would use a 5 M Tris solution to prepare 200 ml of each of the following solutions: 1M, 100 mM ,10 mM , 1 mM, 0.1 mM.

How much of the first dilution should you make to perform the following dilutions?

19. 300 ml of a 1:5 dilution

20. 100 ml of a 1:10 dilution

21. What is the minimum volume you should pipette with the following? (include units with your answers):

P2 P10 P20 P100 P200 P1000

22. How accurate was your pipetting?

23. Was it better with the larger volume or smaller volume pipettor? Provide rationale.

(If there was a consistent problem with one pipettor please tell the instructor.)

Standard Curves

24. Complete a standard curve for the data above and tape a printed version into your notebook.

25. What is the R-squared value of your trend line? Is this good or bad… i.e. would you repeat the assay?

26. What is the protein concentration of (Explain/show your calculations.)

Unknown #1 = Unknown #2 =

Data for DNA Concentration Procedure

Attach a labeled printout of the plate reader raw data as well as your generated standard curve to this lab.

26. What is the concentration of your unknown DNA sample?

27. What is the R 2 for your standard curve data? Why is it important that the R2 is >0.95?

28. Comment on the purity of your DNA... discuss- How can you determine the purity of DNA? What are common contaminants of DNA purified from cells?

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