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23 Jan 2018

- why might it difficult to express an intact human protein coding gene inserted in to bacterial cell?

please the answer should be in advanced molecular genetics

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Lelia Lubowitz
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25 Jan 2018
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Related questions

1.A molecular biologist isolated a gene from a human lung cell and inserted it into a bacterium’s genome. The protein synthesized from this gene was useless because it contained many more amino acids than the protein produced by the lung cell. Explain this observation.

2. What is the CTD? What role does the CTD play in transcription?

Question 1)

Making genetically engineered catfish would be easier than making genetically engineered cows at what step?

A) modfiying the transgene

B) collecting fertilized eggs.

C) producing offspring once a fertilized egg with a transgene introduced into the cell is obtained

D) both b and c above

Question 2)

A transgene designed to be expressed in milk and encode a bacterial protein would:

A) have the bacterial gene promoter and coding region
B) would need to be placed in just the mammary cells

C) have a promoter from a human mammary cell specific gene and the coding region from the bacteria gene

D) have a promoter from a human mammary cell specific gene and the coding region from this same gene.

Question 3)

What is the part of the gene that is the on/off signal?

A) termination sequence

B) promoter

C) coding region

D) Trick question, genes do not need to be signaled to turn on or off. Every gene is always turned on in every cell.

Bacterial cells are used to express proteins from other organisms. Your lab has isolated a human gene that encodes an enzyme that want to produce in a bacterial cell. You can stably transfer the human gene, along with its promoter into a bacterial cell but no human protein is made. Assume that you’ve done the procedure correctly and have established that the sequence you’ve used is the reason for the lack of protein. How can this be explained? 1.5 pts

Once you solved this initial problem, you can detect RNA from the human gene in the bacterial culture. However, the mRNA is twice as long as the mRNA isolated from this gene in humans and the enzyme coded for by the gene does not appear to be functional. Explain why. 1.5 pts

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