What happens when RNA polymerase hits a nonsense mutation?
a. Transcription stops because nonsense is the same as stop.
b. Translation stops because nonsense encodes stop.
c. Transcription continues until a transcription termination site is reached.
d. Replication con5inues until a ter site is reached which terminates replication. e. none of the above
9. A frame-shift mutation
a. can cause premature translation termination.
b. can be caused by the addition or removal of a base pair. c. can cause the production of a truncated protein.
d. aandc
e. all of the above
10. PCR, polymerase chain reaction requires: a. an RNA template.
b. a thermo-tolerant DNA polymerase.
c. primers that have a 3â- Phosphate.
d. dideoxy nucleotide triphosphates for polymerization. e. bandc
1/ Some bacteria use _______ enzymes as a defense mechanism; these enzymes cut up foreign _______.
a. modification;DNA
b. restriction; DNA
c. restriction; RNA
d. modification: Protein e. modification; RNA
2/ The addition of _______ groups to their DNA is one method used by bacteria to prevent their own DNA from being cut by restriction enzymes.
a. phosphate
b. acetly
c. methyl
d. nicatinamide e. adenyl
3/ Which of the following is a palindromic recognition sequence? a. 5 Ì. . . CAATAG . . . 3 Ì
b. 5 Ì. . . CAATTG . . . 3 Ì
c. 5 Ì. . . CATTTG . . . 3 Ì
d. 5 Ì. . . GATTTC . . . 3 Ì e. 5 Ì. . . CATCAT . . . 3 Ì
4/ DNA, because it has a _______ charge, moves to the _______ end of the field in gel electrophoresis; _______ DNA molecules migrate the most quickly.
a. positive; positive; smaller
b. positive; positive; larger
c. positive; negative; smaller
d. negative; positive; larger e. negative; positive; smaller
5. A 10 base-pair single-stranded DNA fragment was cut by three different restriction enzymes to produce the following fragments:
(1) AC, TAC, and GGATG
(2) TA, CGG, and ATGAC
(3) GAC, GAT, and TACG
The nucleotide sequence of the ten-base fragment is a. ATGCCTACTG.
b. TACGGATGAC.
c. GGATGACTAC.
d. GACGATTACG.
e. The sequence cannot be determined from the information given.
What happens when RNA polymerase hits a nonsense mutation?
a. Transcription stops because nonsense is the same as stop.
b. Translation stops because nonsense encodes stop.
c. Transcription continues until a transcription termination site is reached.
d. Replication con5inues until a ter site is reached which terminates replication. e. none of the above
9. A frame-shift mutation
a. can cause premature translation termination.
b. can be caused by the addition or removal of a base pair. c. can cause the production of a truncated protein.
d. aandc
e. all of the above
10. PCR, polymerase chain reaction requires: a. an RNA template.
b. a thermo-tolerant DNA polymerase.
c. primers that have a 3â- Phosphate.
d. dideoxy nucleotide triphosphates for polymerization. e. bandc
1/ Some bacteria use _______ enzymes as a defense mechanism; these enzymes cut up foreign _______.
a. modification;DNA
b. restriction; DNA
c. restriction; RNA
d. modification: Protein e. modification; RNA
2/ The addition of _______ groups to their DNA is one method used by bacteria to prevent their own DNA from being cut by restriction enzymes.
a. phosphate
b. acetly
c. methyl
d. nicatinamide e. adenyl
3/ Which of the following is a palindromic recognition sequence? a. 5 Ì. . . CAATAG . . . 3 Ì
b. 5 Ì. . . CAATTG . . . 3 Ì
c. 5 Ì. . . CATTTG . . . 3 Ì
d. 5 Ì. . . GATTTC . . . 3 Ì e. 5 Ì. . . CATCAT . . . 3 Ì
4/ DNA, because it has a _______ charge, moves to the _______ end of the field in gel electrophoresis; _______ DNA molecules migrate the most quickly.
a. positive; positive; smaller
b. positive; positive; larger
c. positive; negative; smaller
d. negative; positive; larger e. negative; positive; smaller
5. A 10 base-pair single-stranded DNA fragment was cut by three different restriction enzymes to produce the following fragments:
(1) AC, TAC, and GGATG
(2) TA, CGG, and ATGAC
(3) GAC, GAT, and TACG
The nucleotide sequence of the ten-base fragment is a. ATGCCTACTG.
b. TACGGATGAC.
c. GGATGACTAC.
d. GACGATTACG.
e. The sequence cannot be determined from the information given.
