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Briefly explain how blue-white screening works. Include the genes and chemicals needed for this to work properly. Which output would you expect when you have cloned a DNA fragment into a plasmid for blue-white screening? Explain all of the controls necessary to ensure that a cloning experiment with blue-white screening was successful

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Lelia LubowitzLv2

7 Sep 2018

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Related questions

1.Which of the following is true of the blue/white screening technique?

Non-recombinant plasmids are white.

In recombinant plasmids, the LacZÎ± is inactivated by the target gene.

The host strain must contain an intact lac operon.

All of the above

2. During the plasmid miniprep procedure, which of the following reagents was used to denature the nucleic acids into their single-stranded forms?

Sodium dodecyl sulfate

NaOH

Potassium Acetate

Isopropanol

3. Quantification of DNA using the Qubit system involved which of the following?

Measurement of visible light absorbance

Measurement of UV light absorbance

Measurement of fluorescence

None of the above

4. Which of the following occurs during the phenol/chloroform extraction technique?

Nucleic acids are denatured to their single-stranded versions.

Proteins are denatured.

The organic solvents each form a layer above and below the aqueous layer at the interface.

All of the above

5.What result would you expect if you omitted the ammonium acetate prior to ethanol precipitation of a DNA sample after a phenol chloroform extraction of a PCR reaction?

More Taq polymerase would be precipitated with the DNA.

Less of the DNA in the sample would be precipitated in the pellet.

More of the dNTPs would be precipitated in the pellet.

The pellet would be too difficult to resuspend in TE buffer.

CELL RESPIRATION LAB

Section:

Type in your answers in complete sentences, be precise and specific to earn full credit, reformat if necessary

Purpose: The purpose of this lab activity is to analyze the effect of exercise on cellular respiration

Experiment 1 Enzymes in Food

Why is cellular respiration necessary for living organisms?

Why is fermentation less effective then respiration?

List the two co-enzymes involved in cellular respiration and describe their role.

How many ATP molecules are produced in
Glycolysis	Citric Acid Cycle	Electron Transport	Fermentation

Table 1. Fermentation by Yeast

Tube	Initial Gas (mm)	Final Gas (mm)	Net Change
1% Glucose Solution
1% Sucrose Solution
1% Equal Solution
1% Splenda Solution
1% Sugar Solution

Include your hypothesis for this experiment. Be specificâ¦in other words we sure to include at least one end point you will measure to support your predictions. Natural sugar causes fermentation by producing CO2

Did you notice a difference in the rate of respiration between the sugars? Did any of the artificial sugars provide a good starting material for fermentation? Yes no artificial sugar did not provide a good starting material for fermentation as there was no change in height of gas.

Which tubes displayed evidence of anaerobic fermentation? How did you know? Due to change in gas height we can determine fermentation was occurring during to production of CO2

Hypothesize why some of the solutions were not metabolized. Research their chemical formulas.

How do the results of this experiment related to the role yeast play in baking?

Experiment 2Aerobic Respiration in Beans

How did the color of the bromothymol blue solution in each beaker change over time in each condition?

What is the mechanism driving the bromothymol blue solution color change?

What can be inferred from the color change of the bromothymol blue solution?

What evidence do you have that cellular respiration occurred in the beans? Explain your answer.

What are the controls in this experiment, and what variables do they eliminate?

If this experiment were conducted at 0 Â°C, what difference would you see in the rate of respiration? Why?

Table 2: Bromothymol Blue Color Change Over Time for Pinto Bean Trial
Time	Pre-Soaked Pinto Beans	Dry Pinto Beans	No Pinto Beans
0 min
30 min
60 min
90 min
120 min
150 min
180 min
24 hours

Table 3: Bromothymol Blue Color Change Over Time for Kidney Bean Trial
Time	Pre-Soaked Kidney Beans	Dry Kidney Beans	No Kidney Beans
0 min
30 min
60 min
90 min
120 min
150 min
180 min
24 hours

Q.In the non-9:3:3:1 ratio dihybrid cross below, there are 3 classes of phenotypes: white, pink, red which of the following is NOT true?

9 A_B_ white

3 A_bb pink

3 aaB_ red

1 aabb red

a.) phenotype is dependent on the genotype of gene B

b.) This is an example of recessive episasis

c.) aa is epistatic to B_, and bb

d.) phenotype is dependent on the genotype of gene A

Q,When choosing a selectable marker, you...

a.) choose a enhancer for your gene of interest

b.) choose a rescue gene to save your gene of interest

c.) choose a resistant gene that will allow for colony screening

d.) choose a gene that encodes for DNA replication

Q.The following steps detail an experiment resulting in transgenic mice with a knockout gene for fur color. Recall that agouti (brown) mice are A/A;M/M and black fur coats are a/a;M/M . List the steps in their correct chronological order.

1.Inject transformed agouti embryotic cells into an a/a;M/M embryo to produce A/A;M/m and a/a;M/M altered embryo

2.Self-cross hemizygous mice to produce homozygous transgenic mice (A/-;M/m)

3.Create a a targeting vector containing tk+ for ganciclovir sensitivity, neoR for neomycin resistance, and the gene encoding fur color.

4.Observe mice with chimeric phenotype and cross them with black a/a;M/M mice to produce transgenic hemizygous progeny (A/a;M/m)

5.Isolate all transformed embryonic stem cells that show homologous recombination and inject them into a embryonic stem cells of an agouti mouse.

a.) 3,5,4,1,2

b.) 1,5,3,4,2

c.) 3,5,1,4,2

d.) 5,3,2,4,2

If dog 1 has is black with alleles (A_B_) , and dog 2 is brown with (aaB_), and dog 3 is yellow with (_ _bb) alleles, what kind of epistasis is this and what color would dog 4 be if he was (AAbb).

1. Recessive epistasis, Yellow

2. Recessive epistasis, Black

3. Dominant epistasis, Yellow

4. Dominant epistasis, Black

A gene interaction in which pair of recessive genes at one locus prevents expression of dominant allele at another locus called

a. incomplete dominance

b. epistatis

c. complete dominance

d. polygenic inheritance