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BCH2011: Textbook summary - Lecture 20 + 21

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Department
Biology
Course
BCH2011
Professor
Various
Semester
Spring

Description
LECTURE 2021 CationExchange Chromatography In cationexchange chromatography the solid matrix has negatively charged groups In the mobile phase proteins with a net positive charge migrate through the matrix more slowly than those with a net negative charge because the migration of the former is retarded more by interaction with the stationary phaseHighPerformance Liquid Chromatography Chromatographic methods are typically enhanced by the use of HPLC orhighperformance liquid chromatography HPL makes use of highpressure pumps that speed the movement of the protein molecule down the column s well as hi
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