MGY299Y1 Lecture Notes - Copy-Number Variation, Hybridization Probe, G Banding
3 Jun 2013
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Document Summary
Constitutional cytogenetics chromosomes born with present since conception. Hypotonic solution swells cells cells explode; chromosomes spread across slide chromosomes look like black bands if use certain enzymes to treat them, digests certain chromosomes away g banding pattern take picture on computer . G-banded chromosomes each chromosome has its own distinctive banding pattern looking for differences in banding pattern between two homologous chromosomes. If extra band in one of the homologs, tells you there is abnormality. Unlike sequencing, with small piece of dna to sequence; this is low-resolution whole view of the genome so can see what"s going on can detect extra copies of chromosome missing not very high- resolution. If a child is missing that much dna in 18 typically cause of child"s condition. To confirm: every time if you look at parents, their chromosomes are normal typically de novo events.
