🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
Biology
9
answers
0
watching
343
views
30 Oct 2018

Which of the following can act as a vector to introduce new genes into a cell?

a.

stem cells
b.

ligase
c.

cDNA
d.

primers
e.

plasmids

Watch
9
answers
0
watching
343
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
4 Apr 2023
Already have an account? Log in
Share
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

How are restriction enzymes used to insert foreign DNA into plasmids?

a. use the same restrcition enzyme to cut the insert and the vector, then ligate them together with DNA ligase

b. use a restriction enzyme to cut the insert into several fragments, then allow the fragments to recombine with the vector

c. use a restriction enzyme to cut the vector into several fragments, then use DNA ligase to reassemble the fragments around the insert.

d. use a unique restriction enzyme to cut the insert and the vector, then use Taq polymerase to extend the sticky ends, followed by treatment with DNA ligase

e. none of these procedures will work

14. An enhancer would be used for which of the following purposes
[A] Constructing a gene library
[B] Constructing an expression vector

[C] Doing a restriction digestion
[D] Attempting to "silence" a gene
[E] Constructing a recombinant plasmid

16. For gene therapy to be most effective, genes should be inserted in _______ cells
[A] white blood
[B] red blood

[C] stem
[D] All of the above

Which of the following is not a step in therapeutic cloning
[A] Eggs are taken from the recipient.
[B] A cell is removed from the recipient.

[C] The egg is stimulated to divide.
[D] Embryonic stem cells form.
[E] Stem cells differentiate into the desired cell types.

28. If a researcher were trying to entice a cell to become a neuron, the best candidate cell to start with would be a(an)
[A] pluripotent cell.
[B] adult stem cell.
[C] totipotent cell.
[D] cell from the bone marrow.

1. In a successful PCR reaction, which of the following is true?

a. The template DNA is added in excess of the primers and dNTPs.

b. The annealing temperature must be just below the melting temperature of the primers.

c. The temperature for the denaturation step is 72 C

d. All of the above

2. In our TLC experiment, which of the following had an effect on the migration of the samples?

a. The number of negatively charged phosphate groups

b. The protonation state of ionizable groups on the bases.

c. The size of the molecule.

d. All of the above

3. Which of the following is true of nucleic acid migration in agarose gel electrophoresis?

a.Nicked circular plasmids migrate faster than supercoiled plasmids.

b.Binding of ethidium bromide during electrophoresis speeds up the movement of linear fragments.

c.A higher percentage of agarose will decrease the migration of larger DNA fragments.

d.A lower strength electric field will increase the migration of DNA fragments.

4.What is the purpose of the outgrowth period during transformation?

a.To allow for the cells to take up DNA.

b.To allow for expression of the lacZα and lacZω genes.

c.To allow for expression of antibiotic-resistance genes.

d.To prepare cell membranes for electroporation.

5.Which of the following is true of ligase and ligation reactions?

a.Ligase facilitates H-bonds between the sticky ends created by restriction enzyme digestion.

b.Ligase is inactivated at 37 C.

c.DNA fragments must have a 5’ PO4 group for ligase to covalently join them together.

d.Vector must be added in molar excess to insert to favor recombinant plasmid formation.

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in