E. coli cells are growing in a medium containing lactose but no glucose. How would a mutation that prevents binding of CAP to its binding site near the Lac promoter affect expression of the Lac operon?
A) increase expression of the lac operon
B) decrease expression of the lac operon
C) not change expression of the lac operon
| E. coli cells are growing in a medium containing lactose but no glucose. How would a mutation that prevents binding of CAP to its binding site near the Lac promoter affect expression of the Lac operon?
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QUESTION 1
In the bacterial lac repressor, which of these occurs: (select all that apply)
| (a)In the presence of glucose and the absence of lactose, the repressor is actively produced. | ||
| (b)The lac operon can be hyper-induced in the presence of low glucose by the 'cAMP binding protein' (CAMP). | ||
| (c)In the presence of lactose and the absence of glucose the lac operon is repressed. | ||
| (d)Allolactose is a byproduct of β-galactosidase, indicating high lactose when it builds up. |
1.5 points
QUESTION 2
In eukaryotes the enhancer elements can be quite a distance upstream or downstream from the promoter, and transcriptional regulation is helped by protein:protein interactions.
True
False
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QUESTION 3
The prokaryotic promoter contains a TATA box that is at -10bp upstream from the transcriptional start site.
True
False
1.5 points
QUESTION 4
During the addition of the poly A tail which of these occurs:(select all that apply)
| (a)The transcript is synthesized beyond the poly A site (AAUAAA). | ||
| (b)The mRNA contains a 5â cap. | ||
| (c)The transcript is cleaved 35nt downstream from the poly A site. | ||
| (d)An enzyme complex containing an exonuclease and polyadenylate polymerase cleaves nearby the AAUAA site. | ||
| (e)100-300 Aâs are added to the 5â end. |