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Biology examins all the life around us, from the human body, down to the smallest microbes. Building on top of the other sciences, biologists study the physical structures, chemical processes, and the evolution mechanisms of the earth as we know it

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OC user
OC user
in Biology·
2 Jan 2018

. You are interested in continuing the work of Dr Mendes. What is one additional question that you might logically ask about this issue? Justify the question that you propose by logically describing why this is an important question to ask. Design and describe how to run an experiment to test the question you proposed. Be very specific. Make sure that the design is feasible (no growing plants on Jupiter). Include the following: · Independent and dependent variables · Control variables · Equipment and supplies needed · Outline the basic protocol to follow. · Describe WHY and HOW you will manipulate variables

here is the full question:

Do soil microorganisms protect against crop disease?

The rhizosphere, the soil layer surrounding plant roots, is a complex community in which archaea, bacteria, fungi, and plants interact with one another. After plants are attacked by fungal or bacterial pathogens, soil from the rhizosphere may protect plants from future attacks. Such protective soil is called disease-suppressive.

Researchers investigated whether microorganisms were responsible for the protective effects of disease-suppressive soils. Disease-suppressive soil was obtained from an agricultural field in the Netherlands in which sugar beet crops had previously been attacked by a fungal pathogen, Rhizoctonia solani. Other soil was collected from the margin of the field. The researchers predicted that soil from the margin would not offer protection against pathogens.

The researchers then planted and raised sugar beets in greenhouses, using 5 different soil treatments. Each soil treatment was applied to 4 pots, and each pot contained 8 plants. The pots were inoculated with Rhizoctonia solani and placed in greenhouses kept at 20°C with a 16-hour light: 8-hour dark cycle. After 20 days, researchers determined the percentage of infected sugar beet seedlings for each soil treatment. The results of the experiment are shown in the table.

Soil Treatment

Percentage of Seedlings

with Fungal Disease

Disease-suppressive soil

3.0

Soil from margin of field

62

Soil from margin of field

+10% disease-suppressive soil

39

Disease-suppressive soil heated

to 50∘C for 1 hour

31

Disease-suppressive soil heated

to 80∘C for 1 hour

70

(Data from R. Mendes, et al. Deciphering the rhizosphere for disease-suppressive bacteria, Science 332: 1097-1100 (2011)).



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